dNTP Mix (10 mM each)
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dNTP Mix (10 mM each)
Thermo Scientific™

dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
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Catalog NumberQuantity
R01921 mL
R01910.2 mL
R01935 x 1 mL
R019410 x 1 mL
Catalog number R0192
Price (USD)
130.65
Online Exclusive
158.00
Save 27.35 (17%)
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Quantity:
1 mL
Request bulk or custom format
Price (USD)
130.65
Online Exclusive
158.00
Save 27.35 (17%)
Each
Add to cart

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM. The nucleotides have greater than 99% purity, are free of nuclease activities, human and E. coli DNA. Mixes offer the ability to reduce the number of pipetting steps and the associated risk of reaction set up errors, and are designed for use in a wide variety molecular biology applications.

Features

  • Greater than 99% purity confirmed by HPLC
  • Free of human and E. coli DNA
  • Long term stability, even after multiple freeze-thaw cycles when properly stored
  • Up to 95% of dNTPs remain in triphosphate form after 7 weeks at room temperature
  • Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C, 3 minutes at 72°C)

Applications

  • Long range PCR (40 kb)
  • cDNA synthesis
  • PCR, RT-PCR, qPCR
  • High fidelity PCR
  • DNA labeling and sequencing
  • LAMP, RDA, MDA
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration10 mM
Purity or Quality Grade>99%
Quantity1 mL
Shipping ConditionDry Ice
For Use With (Application)Standard PCR, High-fidelity PCR, RT-PCR, Real Time PCR (qPCR)
FormLiquid
Label or DyeUnlabeled
Unit SizeEach
Contents & Storage
dNTP Mix (10 mM) contains premixed dATP, dCTP, dGTP and dTTP at a final concentration of 10 mM (1 mL)

Store at –20°C.

Frequently asked questions (FAQs)

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

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