ROX Solution, 50 μM
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Thermo Scientific™

ROX Solution, 50 μM

Thermo Scientific ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of theRead more
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Catalog NumberQuantity
R13711 mL
Catalog number R1371
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136.00
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1 mL
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Price (USD)
136.00
Each
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Thermo Scientific ROX dye is added to the qPCR reactions to serve as an internal reference for normalization of the fluorescent signal when using real-time instruments that can detect ROX. ROX allows for the correction of well-to-well variation appearing due to pipetting inaccuracies and fluorescence fluctuations. It can be added to an entire 2X master mix tube or to an individual reaction mixture (for recommended amounts of ROX Solution for a specific real-time instrument refer to the qPCR manual).

ROX does not participate in PCR and does not interfere with the specificity or sensitivity of qPCR assays.

ROX Solution contains ROX dye as a 50 μM aqueous solution at pH 7.6. The excitation maximum and emission maximum of ROX are at 580 nm and 621 nm, respectively.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration50 μM
Fidelity (vs. Taq)2 X
FormatTube
Product LineROX
Product TypeqPCR Reagents
Quantity1 mL
Volume1 mL
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
Label or DyeROX
PCR MethodqPCR
Unit SizeEach
Contents & Storage
Contains:
• 1 mL ROX Solution, 50 μM

Store at -20°C.
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Frequently asked questions (FAQs)

Is ROX passive reference dye required for fluorescent signal normalization with the PikoReal instrument?

No, neither ROX nor any other passive reference dye is required when using PikoReal. There is no disadvantage if it is present in the reaction mix, but the software will not use their fluorescence data for normalization of the fluorescence signal of the reporter dyes.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3264932Certificate of AnalysisJun 19, 2025R1371
3254287Certificate of AnalysisJun 04, 2025R1371
3237540Certificate of AnalysisMay 13, 2025R1371
3183949Certificate of AnalysisMar 05, 2025R1371
3183948Certificate of AnalysisMar 05, 2025R1371
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