Zeocin™ Selection Reagent
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Zeocin™ Selection Reagent
Gibco™

Zeocin™ Selection Reagent

Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus.
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Catalog NumberQuantity
R250018 x 1.25 mL
R2500550 mL
Catalog number R25001
Price (USD)
343.65
Online Exclusive
380.00
Save 36.35 (10%)
Each
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Quantity:
8 x 1.25 mL
Price (USD)
343.65
Online Exclusive
380.00
Save 36.35 (10%)
Each
Add to cart

Gibco Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. It acts by binding to and cleaving DNA, leading to cell death, and is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. Working concentration range is 50–400 μg/mL depending on the cell type. This product is supplied at 100 mg/mL in sterile water in 8 x 1.25 mL vials.

  • Gibco™ Zeocin™ Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus
  • Zeocin™ Selection Antibiotic acts by binding to and cleaving DNA, leading to cell death
  • It is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures
  • Working concentration ranges between 50–400 μg/mL depending on the cell type
  • This product is supplied at 100 mg/mL in sterile water, in 8 x 1.25 mL vials
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 mg/mL
Culture TypeMammalian Cell Culture, Insect Cell Culture
For Use With (Application)Eukaryotic Selection/Stable Cell Line Generation, Bacterial Selection
Product LineZeocin™
Quantity8 x 1.25 mL
FormLiquid
Product TypeAntibiotic
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: -10 to -30°C
Shipping conditions: Ice

Frequently asked questions (FAQs)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

I received Zeocin and it looks blue. Is this normal?

Zeocin is a formulation of phleomycin D1, a basic, water-soluble, copper-chelated glycopeptide isolated from Streptomyces verticillus. The presence of copper gives the solution its blue color. This copper-chelated form is inactive. When the antibiotic enters the cell, the copper cation is reduced from Cu2+ to Cu1+ and removed by sulfhydryl compounds in the cell. Upon removal of the copper, Zeocin is activated and will bind DNA and cleave it, causing cell death.

What is the molecular weight of Zeocin?

MW=1,535. Molecular formula: C60H89N21O21S3.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What safety precautions should be taken when autoclaving Zeocin-containing media and plates before disposal? Are there any long-term effects for people who are exposed to Zeocin on a daily basis?

Zeocin, in media or other liquid form, is a health hazard when it comes in contact with your skin or upon ingestion. When it is in the powder solid form, it can become a respiratory hazard as well as contact and ingestion hazard. If the liquid or media is placed in an autoclavable bag, it can be autoclaved. The elevated temperatures within the autoclave will inactivate Zeocin. Sodium hypochloride (bleach) will also inactivate Zeocin. We highly recommend wearing the appropriate personal protective equipment (lab coat, gloves, and eye protection) when handling Zeocin.

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