Zeocin™ Selection Reagent
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Zeocin™ Selection Reagent
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Gibco™

Zeocin™ Selection Reagent

Gibco™ Zeocin™ Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. Zeocin™ Selection AntibioticRead more
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Catalog NumberQuantity
R2500550 mL
R250018 x 1.25 mL
Catalog number R25005
Price (USD)
1,256.65
Online Exclusive
1,390.00
Save 133.35 (10%)
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Quantity:
50 mL
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Price (USD)
1,256.65
Online Exclusive
1,390.00
Save 133.35 (10%)
Each
Add to cart
Ask our AI about this Product
Gibco™ Zeocin™ Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. Zeocin™ Selection Antibiotic acts by binding to and cleaving DNA, leading to cell death.

Zeocin™ Selection Antibiotic is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. It is used at a working concentration ranging between 50–400 μg/mL depending on the cell type. This product is supplied at 100 mg/mL in sterile water in one 50 mL bottle.

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.

Zeocin is a trademark of Invivogen.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 mg/mL
Culture TypeMammalian Cell Culture, Insect Cell Culture
For Use With (Application)Eukaryotic Selection/Stable Cell Line Generation, Bacterial Selection
Product LineZeocin™
Quantity50 mL
FormLiquid
Product TypeAntibiotic
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: -5 to -20°C
Shipping conditions: Ice
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Frequently asked questions (FAQs)

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

Zeocin is a formulation of phleomycin D1, a basic, water-soluble, copper-chelated glycopeptide isolated from Streptomyces verticillus. The presence of copper gives the solution its blue color. This copper-chelated form is inactive. When the antibiotic enters the cell, the copper cation is reduced from Cu2+ to Cu1+ and removed by sulfhydryl compounds in the cell. Upon removal of the copper, Zeocin is activated and will bind DNA and cleave it, causing cell death.

MW=1,535. Molecular formula: C60H89N21O21S3.

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

Zeocin, in media or other liquid form, is a health hazard when it comes in contact with your skin or upon ingestion. When it is in the powder solid form, it can become a respiratory hazard as well as contact and ingestion hazard. If the liquid or media is placed in an autoclavable bag, it can be autoclaved. The elevated temperatures within the autoclave will inactivate Zeocin. Sodium hypochloride (bleach) will also inactivate Zeocin. We highly recommend wearing the appropriate personal protective equipment (lab coat, gloves, and eye protection) when handling Zeocin.

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