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Citations & References (29)
Invitrogen™
CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent
CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation ofRead more
| Catalog Number | Quantity |
|---|---|
| R37111 | 1.75 mL kit |
Catalog number R37111
Price (USD)
388.00
Each
Quantity:
1.75 mL kit
Price (USD)
388.00
Each
CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent™ Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death.
• Simple and fast no-wash protocol helps preserve delicate apoptotic cells
• Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.
See other ReadyProbes™ reagents for cell staining
Learn more about other assays for apoptosis
Cell imaging applications
CellEvent™ Caspase-3/7 Green reagent is a four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is non-fluorescent when not bound to DNA. The CellEvent™ Caspase-3/7 Green reagent is intrinsically non-fluorescent, as the DEVD peptide inhibits binding of the dye to DNA. Upon activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved and the free dye can bind DNA, generating a bright green fluorescence. The fluorescence emission of the dye when bound to DNA is 530 nm and can be observed using a standard FITC filter set.
Suggestions for use
• In most cases, 2 drops/ml and an incubation time of 30 minutes is sufficient for bright nuclear staining of apoptotic cells; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• If desired, combine CellEvent™ Caspase-3/7 Green ReadyProbes™ reagent with a red or far-red nuclear stain for a total cell count
• CellEvent™ Caspase-3/7 Green dye is excited with a maximum at 502 nm and has an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.
• Simple and fast no-wash protocol helps preserve delicate apoptotic cells
• Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area.
See other ReadyProbes™ reagents for cell staining
Learn more about other assays for apoptosis
Cell imaging applications
CellEvent™ Caspase-3/7 Green reagent is a four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is non-fluorescent when not bound to DNA. The CellEvent™ Caspase-3/7 Green reagent is intrinsically non-fluorescent, as the DEVD peptide inhibits binding of the dye to DNA. Upon activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved and the free dye can bind DNA, generating a bright green fluorescence. The fluorescence emission of the dye when bound to DNA is 530 nm and can be observed using a standard FITC filter set.
Suggestions for use
• In most cases, 2 drops/ml and an incubation time of 30 minutes is sufficient for bright nuclear staining of apoptotic cells; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• If desired, combine CellEvent™ Caspase-3/7 Green ReadyProbes™ reagent with a red or far-red nuclear stain for a total cell count
• CellEvent™ Caspase-3/7 Green dye is excited with a maximum at 502 nm and has an emission maximum at 530 nm. It is detected through standard GFP and FITC filters.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Compatible CellsMammalian Cells
Excitation/Emission502/530
For Use With (Application)Cell Imaging
For Use With (Equipment)Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
Label TypeOther Label(s) or Dye(s)
Product LineCellEvent, ReadyProbes
Product TypeCaspase 3/7 Reagent
Quantity1.75 mL kit
Detection MethodFluorescence
FormatDropper Bottle
Unit SizeEach
Contents & Storage
1 × 1.75 mL dropper bottles
Store at ≤ 25°C.
Store at ≤ 25°C.
Frequently asked questions (FAQs)
How many tests can I perform with each Invitrogen CellEvent Caspase-3/7 Green ReadyProbes product?
How should Invitrogen CellEvent Caspase-3/7 Green ReadyProbes Reagents be stored, and what formats are supplied?
Can cleaved Invitrogen CellEvent Caspase-3/7 Detection Reagents stain cytoplasmic RNA?
How long can Invitrogen CellEvent Caspase Detection Reagents remain in continuous culture?
Can I use a nuclear counterstain with Invitrogen CellEvent Caspase Detection Reagents?
Citations & References (29)
Citations & References
Abstract
SHOX triggers the lysosomal pathway of apoptosis via oxidative stress.
Journal:
PubMed ID:24186869
The SHOX gene encodes for a transcription factor important for normal bone development. Mutations in the gene are associated with idiopathic short stature and are responsible for the growth failure and skeletal defects found in the majority of patients with Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia. SHOX is expressed
Disruption of mitochondrial quality control genes promotes caspase-resistant cell survival following apoptotic stimuli.
Journal:J Biol Chem
PubMed ID:35304098
Design and synthesis of novel 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4-thiadiazol- 2-yl)urea derivatives with potent anti-CML activity throughout PI3K/AKT signaling pathway.
Journal:Bioorg Med Chem Lett
PubMed ID:31097376
'In this investigation, a series of 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4- thiadiazol-2-yl)urea receptor tyrosine kinase inhibitors were synthesized by a simple and efficient structure-based design. Structure-activity relationship (SAR) analysis of these compounds based on cellular assays led to the discovery of a number of compounds that showed potent activity against human chronic myeloid leukemia
SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial ?? in neuroblastoma cells.
Journal:Oncol Lett
PubMed ID:28599461
'Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27)'
The Development of a Nano-based Approach to Alleviate Cisplatin-Induced Ototoxicity.
Journal:J Assoc Res Otolaryngol
PubMed ID:29349595
'Cisplatin-induced hearing loss is experienced by a high percentage of patients with squamous cell carcinoma undergoing cisplatin chemotherapy. A novel nano-construct capable of sequestering extracellular cisplatin was developed to combat this problem. The nano-construct consisted of superparamagnetic iron oxide nanoparticles (SPIONs) entrapped within polymeric micelles, which were formed from a