PathoDX™ Strep Grouping Kit

Use Thermo Scientific™ Remel™ PathoDx™ Strep Grouping kit to perform a latex agglutination test designed to identify beta-hemolytic streptococci of Lancefield Groups A, B, C, F and G from primary culture plates. The PathoDx procedure utilizes a latex agglutination method in conjunction with a nitrous acid extraction procedure^1,2. The IgG coupled to the latex is highly specific for a given streptococcal group antigen. This method offers significant advantages over other streptococcal grouping procedures in terms of rapidity, simplicity, and convenience.

The streptococcal group carbohydrates of streptococcal groups A, B, C, F and G are complex antigens with a rhamnose oligosaccharide and differing side chains, consisting primarily of glucosamine, either acetylated or non-acetylated. In the PathoDx procedure, specific antibody on latex particles reacts with, and agglutinates, streptococcal group antigen extracted from the bacterial cell wall. The test is intended for use with streptococcal colonies that are beta-hemolytic on sheep blood agar or for use with pure streptococcal isolates in broth cultures. The reagents are designed to give a positive (2+ or greater) agglutination with one to two 18 to 24 hour colonies for most beta-hemolytic streptococcal isolates.

• Ready-to-use – Prepared reagents
• Easy to interpret – The PathoDx Strep Grouping kit is designed to give a 2+ or greater agglutination reaction with the extract of one or two colonies of an 18 to 24 hour culture of streptococci of Lancefield groups A, B, C and G (large colony variety) in 60 seconds for most streptococcal isolates. Minute colony Group F and small colony strains of other groups require many more colonies (heavy sweep) to give a positive agglutination reaction. A negative test produces a uniform milky appearance with no agglutination after 60 seconds.

The Grouping Latexes are presented as five dropper bottles, one specific for each of the groups A, B, C, F and G, each containing 3.0mL of a suspension of synthetic blue latex particles coated with rabbit antibody IgG-sensitized with 0.098% sodium azide and 0.05% ProClin 300^® as preservatives.

The PathoDx Strep Grouping kit may also be used with beta-hemolytic streptococci grown in broth in pure culture. The kit contents the following reagents:

• Strep A Grouping Latex (R62030) - 1 dropper bottle (red cap)
• Strep B Grouping Latex (R62031) - 1 dropper bottle (pink cap)
• Strep C Grouping Latex (R62032) 1 dropper bottle (orange cap)
• Strep F Grouping Latex (R62034) - 1 dropper bottle (blue cap)
• Strep G Grouping Latex (R62035) - 1 dropper bottle (purple cap)
• Positive Control (R24042) - 1 dropper bottle (yellow cap)
• Reagent 1 (R62050) - 1 dropper bottle (red cap)
• Reagent 2 (R62055) - 1 dropper bottle (blue cap)
• Reagent 3 (R62060) - 1 dropper bottle (green cap)
• Stirrers - 1 bag
• Disposable slides (R62070)- 1 pack

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.