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Ni-NTA Agarose
Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.Read more
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| Catalog Number | Quantity |
|---|---|
| R90115 | 25 mL |
| R90101 | 10 mL |
| R90110 | 100 mL |
Catalog number R90115
Price (USD)
459.65
Online Exclusive
489.00Save 29.35 (6%)
Each
Quantity:
25 mL
Price (USD)
459.65
Online Exclusive
489.00Save 29.35 (6%)
Each
Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine. One-step purification can be performed under both native and denaturing conditions. Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity25 mL
Stationary PhaseNi-NTA
Column TypeAffinity Column
FormSuspension
TypeAgarose
Unit SizeEach
Contents & Storage
The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.