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Invitrogen
Human RFPL2 (aa 268-297) Control Fragment Recombinant Protein
Product Details
RP-105619
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
IQLTTELGFWTVSLRDGGRLSATTVPLTFL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Unconjugated
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (50%), Rat (50%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65510. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
RFPL1, RFPL2 and RFPL3 (ret finger protein-like 1, 2 and 3, respectively), exist as a cluster of genes mapping to human chromosome 22q12.2-q13.3, sharing 95%-96% identity. RFPL1, 2 and 3, are thought to contribute to neocortex organization and size in primates, and show high expression in fetal neocortex as well as embryonic stem-cell neurogenesis. Each of the three RFPL genes encodes two exons giving rise to a putative RING-like motifs and B30-2 domains. RFPL2, or RNF79, is 378 amino acids long and is also highly expressed in prostate with lower expression in fetal kidney and fetal liver. As a result of alternative splicing, two isoforms of RFPL2 exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ret finger protein-like 2; RING finger protein 79
Gene Aliases: RFPL2; RNF79
UniProt ID: (Human) O75678
Entrez Gene ID: (Human) 10739
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