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Invitrogen
Human PI4KA (aa 1858-1925) Control Fragment Recombinant Protein
Product Details
RP-108050
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYG
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-67389. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
PI4KB and PI4KA are the two human type III PI4 kinases, which share sequence similarly with PI3 kinases and phosphorylate the 4 position of phosphotidyl inositol. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by PI4KA is a type III enzyme that is not inhibited by adenosine. Two transcript variants encoding different isoforms have been described for PI4KA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: phosphatidylinositol 4-kinase; phosphatidylinositol 4-kinase 230; Phosphatidylinositol 4-kinase alpha; Phosphatidylinositol 4-Kinase III alpha; phosphatidylinositol 4-kinase, catalytic, alpha; phosphatidylinositol 4-kinase, type III, alpha; PI4-kinase alpha; ptdIns-4-kinase alpha; testicular secretory protein Li 35
Gene Aliases: PI4K-ALPHA; pi4K230; PI4KA; PIK4; PIK4CA; PMGYCHA
UniProt ID: (Human) P42356
Entrez Gene ID: (Human) 5297
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