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Product Details
RP-94398
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
MEENNLQCSSVVDGNFEEVPRETAIQFKPPLYRQRYQFVKNLVDQHEPKKVADLGCGDTSLLRLLKVNPCIELLVGV
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Unconjugated
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (68%), Rat (68%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-55866. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
HENMT1 (HEN Methyltransferase 1) is a small RNA methyltransferase gene crucial for the stability and functionality of PIWI-interacting RNAs (piRNAs). It is located in human chromosome 1 at the p36.31 locus. The HENMT1 protein catalyzes the 2'-O-methylation at the 3' end of piRNAs in a Mg2+ dependent manner. This modification is essential for piRNA maturation and stability, ensuring the repression of transposable elements (TEs) during spermatogenesis and maintaining genomic integrity in male germ cells. Studies have highlighted the impact of HENMT1 mutations causing male infertility due to disrupted piRNA pathway and improper germ cell gene regulation. The enzymatic activity of HENMT1 is not only significant for male fertility but is also involved in maintaining piRNA stability in female germ cells, although females with HENMT1 mutations show subfertility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HEN1 methyltransferase homolog 1; hua enhancer 1 homolog 1; Small RNA 2'-O-methyltransferase
Gene Aliases: C1orf59; HEN1; HENMT1
UniProt ID: (Human) Q5T8I9
Entrez Gene ID: (Human) 113802
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