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Invitrogen
Human C16orf57 (aa 185-262) Control Fragment Recombinant Protein
Product Details
RP-98621
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
LVSEVDRVMEEFNLTTFYQDPSFHLSLAWCVGDARLQLEGQCLQELQAIVDGFEDAEVLLRVHTEQVRCKSGNKFFSM
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Unconjugated
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (85%), Rat (85%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59732. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
C16orf57 is a phosphodiesterase responsible for the U6 snRNA 3' end processing. This protein acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA 3' end-terminated with a 2',3'-cyclic phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' RNA exonuclease USB1; FLJ13154; hUsb1; HVSL motif containing 1; Mutated in PN protein 1; Mutated in poikiloderma with neutropenia protein 1; putative U6 snRNA phosphodiesterase; U six biogenesis 1; U6 snRNA biogenesis 1; U6 snRNA phosphodiesterase 1; UPF0406 protein C16orf57; USB1
Gene Aliases: C16orf57; hUsb1; HVSL1; Mpn1; PN; USB1
UniProt ID: (Human) Q9BQ65
Entrez Gene ID: (Human) 79650
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