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Invitrogen
Human PAF49 (aa 154-259) Control Fragment Recombinant Protein
Product Details
RP-98992
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GNPPVTGPRSALAPNLLTSGKKKKEMQVTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLEPLGVLFPSTTKKRKKPKGK
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (44%), Rat (44%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83573. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.; Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A34.5; antis; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3e antigen, epsilon polypeptide associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; DNA-directed RNA polymerase I subunit G; DNA-directed RNA polymerase I subunit RPA34; RNA polymerase I-associated factor PAF49
Gene Aliases: ASE-1; ASE1; CAST; CD3EAP; PAF49; POLR1G
UniProt ID: (Human) O15446
Entrez Gene ID: (Human) 10849
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