These cryopreserved purified Kupffer cells are isolated from adult male Sprague-Dawley rats. Each vial contains a minimum of one million cells.

Easy to use cryopreserved Kupffer cells provide a convenient way to produce custom cell ratios for hepatic co-culture.

Each lot of Cryopreserved Rat Kupffer Cells is tested for:

• Purity
• Response to activation with LPS
• Adherence
• Minimum 1 million cells per vial

Kupffer Cells for Improved Physiological Modeling
Growing evidence shows that under both normal and pathological conditions, many hepatocyte functions are regulated by substances released from neighboring non-parenchymal cells (NPC). These cells, particularly Kupffer cells, play an important role in the modulation of xenobiotic metabolism in the liver. Kupffer cells secrete potent mediators of the inflammatory response that controls liver inflammation. These cytokine mediators control hepatocyte metabolic rates through direct interactions with phase I and phase II enzymes. Kupffer cell and hepatocyte co-cultures can self-assemble within 72 hours of treatment with pro-inflammatory cytokines or LPS, and can function by effectively modulating P450 expression, thus giving a more physiologically relevant result. Studies indicate that co-culture of hepatocytes with NPCs would better represent both normal liver physiology as well as disease states.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.,

Note:
These products require shipment in LN₂ vapor dewars therefore additional fees may apply.