Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Color | Green |
Concentration | 5 mM |
Excitation Wavelength Range | RNA: 486 nm DNA: 485 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 100 μL |
Detection Method | Fluorescence |
Description | SYTO 9 Green Fluorescent Nucleic Acid Stain |
Emission | RNA: 501 nm DNA: 498 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 100 µL |
Color | Green |
Concentration | 5 mM solution in DMSO |
Excitation Wavelength Range | DNA: 508 nm RNA: 510 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 250 μL |
Detection Method | Fluorescence |
Description | SYTO 11 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO |
Emission | DNA: 527 nm RNA: 530 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 250 µL |
Color | Green |
Concentration | 4 mM solution in DMSO |
Excitation Wavelength Range | RNA: 499 nm DNA: 500 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 315 μL |
Detection Method | Fluorescence |
Description | SYTO 12 Green Fluorescent Nucleic Acid Stain, 4 mM Solution in DMSO |
Emission | RNA: 522 nm DNA: 519 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 315 µL |
Color | Green |
Concentration | 5 mM solution in DMSO |
Excitation Wavelength Range | RNA: 491 nm DNA: 488 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 250 μL |
Detection Method | Fluorescence |
Description | SYTO 13 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO |
Emission | RNA: 514 nm DNA: 509 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 250 µL |
Color | Green |
Concentration | 5 mM solution in DMSO |
Excitation Wavelength Range | RNA: 517 nm DNA: 521 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 250 μL |
Detection Method | Fluorescence |
Description | SYTO 14 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO |
Emission | RNA: 549 nm DNA: 547 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 250 µL |
Color | Green |
Concentration | 1 mM solution in DMSO |
Excitation Wavelength Range | RNA: 494 nm DNA: 488 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 250 μL |
Detection Method | Fluorescence |
Description | SYTO 16 Green Fluorescent Nucleic Acid Stain, 1 mM Solution in DMSO |
Emission | RNA: 525 nm DNA: 518 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 250 µL |
Color | Green |
Concentration | 5 mM solution in DMSO |
Excitation Wavelength Range | DNA: 494 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 250 μL |
Detection Method | Fluorescence |
Description | SYTO 21 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO |
Emission | DNA: 517 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 250 µL |
Color | Green |
Concentration | 5 mM solution in DMSO |
Excitation Wavelength Range | DNA: 490 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 250 μL |
Detection Method | Fluorescence |
Description | SYTO 24 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO |
Emission | DNA: 515 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 250 µL |
Color | Green |
Concentration | 5 mM |
Excitation Wavelength Range | RNA: 487 nm DNA: 485 nm |
Dye Type | SYTO™ BC Green |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Quantity | 100 μL |
Volume (Metric) | 100 μL |
Detection Method | Fluorescence |
Description | SYTO BC Green Fluorescent Nucleic Acid Stain |
Emission | RNA: 504 nm DNA: 500 nm |
Form | Solution |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Solubility | DMSO (Dimethylsulfoxide) |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 100 µL |
Color | Green |
Concentration | 5 mM solution in DMSO |
Excitation Wavelength Range | RNA: 490 nm |
Dye Type | Cell-Permeant |
For Use With (Application) | Fluorescent Labeling, Live Cell Imaging, Microarray |
For Use With (Equipment) | Fluorescence Microscope, High Content Imager, Flow Cytometer |
Volume (Metric) | 100 μL |
Detection Method | Fluorescence |
Description | SYTO RNASelect Green Fluorescent Cell Stain, 5 mM Solution in DMSO |
Emission | RNA: 530 nm |
Product Line | SYTO™ |
Shipping Condition | Room Temperature |
Label Type | Fluorescent Dye |
Product Type | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids |
Unit Size | 100 µL |
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S34854 | 100 µL | SYTO 9 Green Fluorescent Nucleic Acid Stain | 345.65 Online price 372.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7573 | 250 µL | SYTO 11 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO | 323.65 Online price 329.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7574 | 315 µL | SYTO 12 Green Fluorescent Nucleic Acid Stain, 4 mM Solution in DMSO | 326.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7575 | 250 µL | SYTO 13 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO | 318.65 Online price 339.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7576 | 250 µL | SYTO 14 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO | 310.65 Online price 343.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7578 | 250 µL | SYTO 16 Green Fluorescent Nucleic Acid Stain, 1 mM Solution in DMSO | 339.65 Online price 345.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7556 | 250 µL | SYTO 21 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO | 323.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S7559 | 250 µL | SYTO 24 Green Fluorescent Nucleic Acid Stain, 5 mM Solution in DMSO | 335.65 Online price 339.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S34855 | 100 µL | SYTO BC Green Fluorescent Nucleic Acid Stain | 347.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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S32703 | 100 µL | SYTO RNASelect Green Fluorescent Cell Stain, 5 mM Solution in DMSO | 354.65 Online price 378.00 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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SYTO Green Fluorescent Nucleic Acid Stains are cell-permeant nucleic acid stains that show a large fluorescence enhancement upon binding nucleic acids. The SYTO dyes can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria.
The SYTO stains share several important characteristics:
• Permeability to virtually all cell membranes, including mammalian cells and bacteria
• High molar absorptivity, with extinction coefficients >50,000 cm-1 M-1 at visible absorption maxima
• Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids
• Quantum yields that are typically >0.4 when bound to nucleic acids.
SYTO Green-Fluorescent Nucleic Acid Stains excitation/emission:
• SYTO 9—RNA 486/501 nm; DNA 485/498 nm
• SYTO 11—RNA 510/530 nm; DNA 508/527 nm
• SYTO 12—RNA 500/519 nm; DNA 499/522 nm
• SYTO 13—RNA 491/514 nm; DNA 488/509 nm
• SYTO 14—RNA 521/547 nm; DNA 517/549 nm
• SYTO 16—RNA 494/525 nm; DNA 488/518 nm
• SYTO 21—DNA 494/517 nm
• SYTO 24—DNA 490/515 nm
• SYTO BC—RNA 487/504 nm; DNA 485/500 nm
• SYTO RNASelect—RNA 490/530 nm
SYTO dyes differ from each other in one or more characteristics, including cell permeability, fluorescence enhancement upon binding nucleic acids, excitation and emission spectra, DNA/RNA selectivity, and binding affinity. The SYTO dyes are compatible with a variety of fluorescence-based instruments that use laser excitation or a conventional broadband illumination source (e.g., mercury- and xenon-arc lamps). Filter sets that are suitable for imaging cells labeled with fluorescein (FITC), Alexa Fluor 488, or GFP will work well for imaging cells stained with SYTO Green Nucleic Acid Stains.
SYTO nucleic acid stains have been used in diverse applications from staining DNA spotted on microarrays to staining live and fixed cells. The SYTO dyes do not act exclusively as nuclear stains in live cells and should not be equated with DNA-selective compounds such as DAPI (Cat. Nos. D1306, D21490) or Hoechst 33342 (Cat. Nos. H1399, H3570), which stain nuclei in live animal cells. Eukaryotic cells incubated with SYTO dyes generally show cytoplasmic or mitochondrial staining, as well as nuclear staining. The SYTO Green Fluorescent Nucleic Acid Stains have proven valuable in a broad range of research applications as well as used in flow cytometry and fluorescence microscopy.
Specific staining applications for some of the SYTO Green Fluorescent Nucleic Acid Stains:
The SYTO 9 stain has been shown to stain live and dead Gram-positive and Gram-negative bacteria. It is a component of the LIVE/DEAD BacLight Bacterial Viability Kits (Cat. Nos. L7007, L7012, L13152).
The SYTO 11 stain (Cat. No. S7573) has been used in conjunction with time-lapse microscopy to examine the cleavage orientation of dividing cells in the developing cerebral cortex.
The SYTO 13 stain (Cat. No. S7575) has been used in combination with propidium iodide (Cat. Nos. P1304, P3566) to monitor glutamate-induced necrosis in cerebellar granule cells.
The SYTO 14 stain (Cat. No. S7576) binds to cytoplasmic RNA, allowing its use in tracking RNA granule transport in living neurons.
Several reports describe the use of SYTO dyes for detecting apoptosis. A series of SYTO nucleic acid stains was screened for the ability to discriminate between apoptotic and non-apoptotic mouse thymocytes, and the SYTO 16 stain (Cat. no. S7578) was found to be optimal for this application. The SYTO 16 stain has also been used with propidium iodide to differentiate live and dead COS-7 cells with a laser-based scanning cytometer.
The SYTO BC Green Fluorescent Nucleic Acid Stain is a mixture of some of our best SYTO dyes for bacterial staining and is a component of the Bacteria Counting Kit (Cat. No. B7277) for bacterial counting in flow cytometry.
The SYTO RNASelect Green Fluorescent Cell Stain is a cell-permeant nucleic acid stain that selectively stains RNA. Although virtually nonfluorescent in the absence of nucleic acids, the SYTO RNASelect stain exhibits bright green fluorescence when bound to RNA (absorption/emission maxima ∼490/530 nm), but only a weak fluorescent signal when bound to DNA. It has been used for staining RNA cargo in exosomes in both live cell samples and isolated exosomes.
SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit
The SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit #1 contains our collection of cell-permeant, green-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 μL each of SYTO 11–14, 16, 21, and 24 dyes.
Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.