iBind™ Fluorescent Detection (FD) Solution Kit
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iBind™ Fluorescent Detection (FD) Solution Kit
Invitrogen™

iBind™ Fluorescent Detection (FD) Solution Kit

The iBind Fluorescent Detection (FD) Solution Kit is intended for use with the iBind Western Device and is optimized forRead more
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Catalog NumberQuantity
SLF10191 kit
Catalog number SLF1019
Price (USD)
119.65
Online Exclusive
124.00
Save 4.35 (4%)
1 kit
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Quantity:
1 kit
Price (USD)
119.65
Online Exclusive
124.00
Save 4.35 (4%)
1 kit
Add to cart
The iBind Fluorescent Detection (FD) Solution Kit is intended for use with the iBind Western Device and is optimized for western blotting with fluorescent-conjugated antibodies, including those commonly used for infrared detection and dual wavelength semi-quantitative analysis. The iBind Fluorescent Detection Solution Kit is optimized with the ideal viscosity for blocking, washing, and antibody dilution.

Learn more about the iBind western systems ›

The iBind Fluorescent Detection (FD) Solution Kit, together with the iBind Western Device and iBind Cards, bring automation and simplicity to your western protocol. Each iBind Fluorescent Detection (FD) Solution Kit contains sufficient solution for 10 mini-membrane reactions. An iBind Western Device and iBind Cards are required for use of this kit.

About the iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind Device wells to an iBind Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Kit Contents1 bottle 5X buffer, 2 vials 100X additive, 1 vial 10% SDS
Sufficient For10 mini-membrane reactions
Detection MethodFluorescence
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineiBind™
Product TypeFluroescent Detection Kit
Quantity1 kit
Unit Size1 kit
Contents & Storage
Contains: 1 bottle 5X buffer, 2 vials 100X additive, 1 vial 10% SDS

Store at 2–8°C.

Frequently asked questions (FAQs)

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What iBind Solution kits are available?

We offer the iBind Solution kit and the iBind Fluorescent Detection (FD) Solution kit. For standard chemiluminescent or chromogenic detection, we recommend the iBind Solution kit (Cat. No. SLF1020) and for fluorescent detection, we recommend the iBind Fluorescent Detection (FD) Solution kit (Cat. No. SLF1019).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all iBind™ Fluorescent Detection (FD) Solution Kit FAQs

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