TetraSpeck™ Microspheres, 0.1 μm, fluorescent blue/green/orange/dark red
TetraSpeck™ Microspheres, 0.1 μm, fluorescent blue/green/orange/dark red
Invitrogen™

TetraSpeck™ Microspheres, 0.1 μm, fluorescent blue/green/orange/dark red

The 0.1 μm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separatedRead more
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Catalog NumberQuantity
T72790.5 mL
Catalog number T7279
Price (EUR)
570,00
0.5 mL
Add to cart
Quantity:
0.5 mL
Price (EUR)
570,00
0.5 mL
Add to cart
Ask our AI about this Product
The 0.1 μm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.

See our full collection of microscope calibration reagents ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibration TypeConfocal Microscope Calibration, Fluorescence Microscope Calibration
FormatSuspension Beads
Product LineTetraSpeck
Quantity0.5 mL
Shipping ConditionRoom Temperature
ColorOrange, Dark Red, Blue, Green
Diameter (Metric)0.1 μm
Product TypeMicrosphere
Unit Size0.5 mL
Contents & Storage
Store in refrigerator 2°C to 8°C and protect from light.
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Figures

Fluorescence spectra

Fluorescence spectra

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3101962Certificate of AnalysisNov 18, 2024T7279
2810839Certificate of AnalysisSep 06, 2024T7279
2593910Certificate of AnalysisJan 05, 2023T7279
2504297Certificate of AnalysisMay 20, 2022T7279
2380403Certificate of AnalysisJul 20, 2021T7279
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Safety Data Sheets

Frequently asked questions (FAQs)

The TetraSpeck Microspheres (Cat. Nos. T7279, T7280, T7281, T7283, T7284, T14792) are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks at 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).

TetraSpeck Blue Dye Spectra
Fluorescence excitation and emission spectra of bead encapsulated TetraSpeck blue dye.
TetraSpeck Blue Dye Spectra

TetraSpeck Orange Dye Spectra


TetraSpeck Green Dye Spectra
TetraSpeck Green Dye Spectra

TetraSpeck Dark Red Dye Spectra
TetraSpeck Dark Red Spectra

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (28)

Citations & References
Abstract
Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging.
Authors:Szymborska A, de Marco A, Daigle N, Cordes VC, Briggs JA, Ellenberg J,
Journal:
PubMed ID:23845946
'Much of life''s essential molecular machinery consists of large protein assemblies that currently pose challenges for structure determination. A prominent example is the nuclear pore complex (NPC), for which the organization of its individual components remains unknown. By combining stochastic super-resolution microscopy, to directly resolve the ringlike structure of the ... More
The cohesion protein ORD is required for homologue bias during meiotic recombination.
Authors:Webber HA, Howard L, Bickel SE
Journal:J Cell Biol
PubMed ID:15007062
'During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along ... More
Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level.
Authors:Ober RJ, Martinez C, Lai X, Zhou J, Ward ES
Journal:Proc Natl Acad Sci U S A
PubMed ID:15258288
'IgG transport within and across cells is essential for effective humoral immunity. Through a combination of biochemical and in vivo analyses, the MHC class I-related neonatal Fc receptor (FcRn) is known to play a central role in delivering IgGs within and across cells. However, little is known about the molecular ... More
Fast, three-dimensional super-resolution imaging of live cells.
Authors:Jones SA, Shim SH, He J, Zhuang X,
Journal:Nat Methods
PubMed ID:21552254
'We report super-resolution fluorescence imaging of live cells with high spatiotemporal resolution using stochastic optical reconstruction microscopy (STORM). By labeling proteins either directly or via SNAP tags with photoswitchable dyes, we obtained two-dimensional (2D) and 3D super-resolution images of living cells, using clathrin-coated pits and the transferrin cargo as model ... More
Practical confocal microscopy and the evaluation of system performance.
Authors:Zucker RM, Price OT
Journal:Methods
PubMed ID:10491274
'The laser scanning confocal microscope has enormous potential in many fields of biology. Currently there is a subjective nature in the assessment of a confocal microscope''s performance by primarily evaluating the system with a specific test slide provided by the user''s laboratory. To achieve better performance from the equipment, it ... More
28 total citations

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