CytoVista™ Tissue Clearing Reagent
CytoVista™ Tissue Clearing Reagent
Invitrogen™

CytoVista™ Tissue Clearing Reagent

CytoVista Tissue Clearing Reagent clears fluorescently labeled tissue to enable sharp and bright 3D fluorescent imaging at any tissue depth.Read more
Have Questions?
Catalog NumberQuantity
V1130030 mL
Catalog number V11300
Price (TWD)
***
Add to cart
Quantity:
30 mL
Price (TWD)
***
Add to cart
Ask our AI about this Product

CytoVista Tissue Clearing Reagent clears fluorescently labeled tissue to enable sharp and bright 3D fluorescent imaging at any tissue depth. This reagent is recommended for tissues that are easier to clear. For tissues that are harder to clear, we recommended the use of CytoVista Tissue Clearing Reagent followed by CytoVista Tissue Clearing Enhancer. CytoVista Tissue Clearing Reagent can also be used as an imaging solution with stable storage of tissues for weeks with minimal change in tissue morphology or brightness, or specificity of the fluorophores. As an imaging solution, it has refractive index of 1.50.

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:

  • Rapid clearing of fluorescently labeled tissue for 3D imaging
  • Minimal shrinkage, expansion, or other morphological changes to tissue
  • Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
  • Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat. No. V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat. No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-μm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-μm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity30 mL
Reagent TypeCell and Tissue Preparation Reagent
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Product LineCytoVista
TypeTissue Clearing Reagent
Contents & Storage
Store at room temperature.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
8WY38Certificate of AnalysisDec 22, 2024V11300
4QS90Certificate of AnalysisAug 20, 2024V11300
9ZG37Certificate of AnalysisMar 14, 2024V11300
5SB72Certificate of AnalysisSep 29, 2023V11300
3PV97Certificate of AnalysisMay 04, 2023V11300
5 results displayed, search above for a specific certificate

Safety Data Sheets

Share catalog number, name or link

1x1 image pixel for data collection