The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ Lentiviral Expression Systems as a positive control to enable the detection of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. This is not a cloning vector.
Advantages • Optimization of viral transduction efficiency • Optimization of 293FT Transfection • 2 day titer of functional virus using EmGFP
Key Features • Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the EmGFP • PKG Promoter for expression of Blasticidin selection marker
Kit Includes pLenti6.2-GW⁄EmGFP Vector
Related SKUs • 293FT Cell Line (R70007; R70007) • ViraPower™ Lentiviral Support Kit (K4970-00) • ViraPower™ Lentiviral Packaging Mix (K4975-00) • Lipofectamine™ 2000 (11668-019; 11668-027)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Constitutive
Delivery Type
Lentiviral
Promoter
CMV
Product Type
Lentiviral Expression Vector
Reporter Gene
GFP (EmGFP)
Selection Agent (Eukaryotic)
Blasticidin
Selection Marker Promoter
PGK Promoter
Protein Tag
Untagged
Cloning Method
Gateway
Quantity
1 Ea.
Vector
pLenti
Product Line
Gateway™, ViraPower™, Vivid Colors™
For Use With (Application)
Reporter Assays, Viral Expression
Contents & Storage
pLenti6.2-GW⁄EmGFP Vector: 20 μg of control vector is supplied in solution as 40 μl of 0.5 μg⁄μl in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. Store at –20°C.