pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:
• FRT site for subsequent targeted integration of a Flp-In™ expression vector Z-Zeocin™ fusion for selection of stable integrants with Zeocin™
These vectors differ in the SV40 promoter that drives expression of the lacZ-Zeocin™ fusion. pFRT/lacZeo2 contains a truncated version of the SV40 promoter. Use of this vector facilitates the isolation of clones that have integrated the vector near enhancer elements in the genome. Selection of stable clones using pFRT/lacZeo2 may allow easier isolation of clones that express a gene of interest at higher levels.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
SV40
Product Type
Flp-In System Expression Vector
Reporter Gene
Beta-Gal (lacZ)
Selection Agent (Eukaryotic)
Zeocin™
Protein Tag
Untagged
Vector
pFRT
Product Line
Flp-In™
For Use With (Application)
Targeted Integration
Contents & Storage
20 μg of vector, either pFRT/lacZeo or pFRT/lacZeo2, is provided supercoiled and lyophilized. Store at -20°C once resuspended. Vectors are guaranteed stable for 6 months when properly stored.