Varioskan™ LUX Multimode Microplate Reader
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Varioskan™ LUX Multimode Microplate Reader
Varioskan™ LUX Multimode Microplate Reader
Varioskan™ LUX Multimode Microplate Reader
Varioskan™ LUX Multimode Microplate Reader
Varioskan™ LUX Multimode Microplate Reader
Varioskan™ LUX Multimode Microplate Reader
Thermo Scientific™

Varioskan™ LUX Multimode Microplate Reader

Analyze a variety of samples with five measurement technologies, including absorbance (UV-Vis), fluorescence intensity, Luminescence, AlphaScreen / AlphaLISA and TRF.
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Catalog NumberMeasurement TechnologyReading OptionsNo. of Dispensers
VLBL00GD0Absorbance, Fluorescence Intensity, LuminescenceTop and BottomNone
VL0000D0Absorbance, Fluorescence IntensityTopNone
VL0L00D0Absorbance, Fluorescence Intensity, LuminescenceTopNone
VL0L0TD0Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTopNone
VLBL00GD1Absorbance, Fluorescence Intensity, LuminescenceTop and Bottom1
VLB000D0Absorbance, Fluorescence IntensityTop and BottomNone
VLBL00GD2Absorbance, Fluorescence Intensity, LuminescenceTop and Bottom2
VLBL00D0Absorbance, Fluorescence Intensity, LuminescenceTop and BottomNone
VLBL0TGD0Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTop and BottomNone
VLBL0TGD1Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTop and Bottom1
VLBLATD0Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreenTop and BottomNone
VLBL0TGD2Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTop and Bottom2
VLBLATGD0Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreenTop and BottomNone
VLBLATGD1Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreenTop and Bottom1
VLBLATGD2Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreenTop and Bottom2
VLBL00D1Absorbance, Fluorescence Intensity, LuminescenceTop and Bottom1
VLBL0TD1Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTop and Bottom1
VLBLATD1Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreenTop and Bottom1
VLBL00D2Absorbance, Fluorescence Intensity, LuminescenceTop and Bottom2
VLBL0TD2Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTop and Bottom2
VLBLATD2Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence, AlphaScreenTop and Bottom2
VLBL0TD0Absorbance, Fluorescence Intensity, Luminescence, Time-resolved FluorescenceTop and BottomNone
Catalog number VLBL00GD0
Price (USD)
-
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Measurement Technology:
Absorbance, Fluorescence Intensity, Luminescence
Reading Options:
Top and Bottom
No. of Dispensers:
None
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Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific™ Varioskan™ LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The instrument simplifies measurement setup with the automatic dynamic range selection, and its smart safety controls ease workflow and help avoid experimental errors. Varioskan LUX raises the bar for reliability and ease, with technology that works in researchers' best interest.

The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.

The Varioskan LUX is a modular, upgradable instrument that provides excellent usability across a variety of applications.

  • Up to five measurement technologies: Absorbance (UV-Vis), Fluorescence intensity (incl. FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA and Time-resolved fluorescence (incl. TR-FRET/hTRF)
  • Filter and monochromator optics
  • Spectral scanning for assay optimization
  • Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
  • Integrated gas control module for setting CO2 and O2 concentrations for cell based assays
  • Simultaneous dispensing and measurement for signal monitoring at the start of a reaction
  • Automatic dynamic range selection, based on signal intensity of each well
  • Smart safety controls designed to identify and alert the user of potential problems before they happen
  • Automated instrument calibration and self-diagnostics for reliable performance
  • Certified HTRF compatible reader by CisBio Bioassays
  • Validated DLReady by Promega Corporation

Varioskan LUX is paired with the powerful and intuitive SkanIt Software, providing excellent usability and flexibility.

  • License free—Each user can install the software on his/her computer
  • Intuitive and flexible interface for multiple users and assays
  • Innovative Virtual Pipetting Tool makes it easy to define samples to plate layout
  • Measurement data is continuously saved to the database, so there's no data lost due to unexpected interruptions such as power outages or accidental aborting
  • Simplified data processing and export
    • Export data in a single mouse click, or have it emailed automatically at the end of the run
    • Several file formats for exporting results: *.xlsx, *.pdf, *.xml, and *.txt
  • Shows real-time spectra and kinetic curves during the measurement
  • Built-in calculations for data analysis (e.g. blank subtraction, average/SD/CV%, standard curve, dose response, quality control, classification, kinetic and spectral analysis, custom formula, etc.)
  • Comprehensive result reports with traceability to runtime actions and settings
  • Two editions available: Research Edition for life science researchers, and Drug Discovery Edition which offers features needed for the FDA 21 CFR Part 11 compliance.

The Varioskan LUX is modular and upgradeable, so you can build the system that best suits your needs. For more information, contact a sales representative.

Specifications
Accuracy (Photometric)0.003 Abs or ±2% at 200 to 399 nm (0 to 2 Abs), 0.003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs)
CO2 Concentration Range0.1 to 15%
CompatibilityAutomation
Depth (English)23 in.
Depth (Metric)58 cm
Dimensions (L x W x H)58 x 53 x 51 cm (23 x 21 x 20 in.)
Dynamic RangeFluorescence intensity: top reading > 6 decades; Luminescence: > 7 decades
Emission FiltersAvailable for purchase
Height (English)20 in.
Height (Metric)51 cm
IncludesSkanIt PC Software, Research Edition
Linearity96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm
Measurement Speed15 s for 96-well Plate, 45 s for 384-well Plate, 135 s for 1536-Well Plate (Minimum Times)
Measurement TypeEndpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
No. of DispensersNone
PrecisionSD <0.001 Abs or CV <0.5%, at 450 nm (0 to 3 Abs)
SensitivityFluorescence intensity (top reading): < 0.4 fmol fluorescein/well (black 384-well plate), Luminescence: < 7 amol ATP/well (white 384-well plate)
Software Language VersionsEnglish, German, French, Spanish, Portuguese, Italian, Japansese, Chinese
Spectral Scanning Speed< 2.2 s/Well 400 to 500 nm, 1 Flash, 2 nm Step
Wavelength RangeAbsorbance: 200-1000 nm; Fluorescence intensity: Excitation: 200-822 nm, Emission: 270-840 nm; Luminescence: 360-670 nm
Weight (English)125 lb
Weight (Metric)57 kg
Width (English)21 in.
Width (Metric)53 cm
Detector TypePhotomultiplier Tube (PMT)
Incubator TemperatureAmbient +4° to 45°C
InterfacePC Software (Thermo Scientific SkanIt Software)
Light SourceXenon Flash Lamp
Measurement TechnologyAbsorbance, Fluorescence Intensity, Luminescence
Plate Type6 to 1536-well Plates (Absorbance 6 to 384-well Plates), μDrop Plates
Product LineVarioskan
Reading OptionsTop and Bottom
Read-out Range0 to 6 Abs
ShakingOrbital
Software TypeSkanIt Software
Wavelength SelectionDouble Monochromator (Absorbance), Double Excitation and Emission Monochromator (Fluorescence Intensity)
Unit SizeEach
skanit-software

Varioskan LUX Multimode Microplate Reader

Fast and reliable results, even for the most challenging of applications.
Download our brochure ›

Featured Smart Notes

  How can built-in smart safety controls help avoid errors in microplate assay experiments?
  How will automatic dynamic range selection ease workflow and improve the outcome of my microplate assays?

software-resource

Thermo Scientific SkanIt Software comes free with your microplate reader purchase and can be installed on as many computers as you like. This easy-to-use program is so intuitive, it requires little-to-no training. SkanIt Software has features that help boost productivity and prevent common errors in the lab.
Learn more ›

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3020-84259Certificate of ComplianceJul 02, 2025VLB000D0
3020-84254Certificate of ComplianceJul 02, 2025VLB000D0
3020-84258Certificate of ComplianceJul 02, 2025VLB000D0
3020-84257Certificate of ComplianceJul 01, 2025VLB000D0
3020-84252Certificate of ComplianceJul 01, 2025VLBL0TD2
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Scientific Resources

Product Information

Frequently asked questions (FAQs)



Power off the Varioskan LUX instrument, open the sliding door, and then press both springs outward (1) to unlock the bottom of the front panel. Tilt the bottom of the panel outwards slightly and lift up to remove.



Look inside the instrument to locate the tray (3). Gently slide the tray to the left and then towards you to access the plate. Check for jams and make sure that the correct holder is installed. When finished, push the tray back into the instrument (exact position doesn’t matter as long as it is completely inside) and reinstall the front panel.

Note: Remove the bottom front cover on the instrument, in order to access the plate carrier, and manually pull out the plate. Do not attempt to grab the plate back through the plate door, otherwise it can cause alignment issues that will require a repair.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We recommend transporting the Varioskan LUX Multimode Microplate Reader in the original packaging it was delivered in, with proper padding in the box, and after securing the tray holder transport lock on the instrument. See page 84 of the user manual for instructions to refit the transport lock of the tray holder.

Note: We also offer a service to move the instrument. Please reach out to serviceproducts@thermofisher.com.

All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

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