XCell4 SureLock™ Midi-Cell
XCell4 SureLock™ Midi-Cell
Invitrogen™

XCell4 SureLock™ Midi-Cell

The XCell4 SureLock Midi-Cell is a midi-gel electrophoresis system that allows you to quickly and easily perform simultaneous vertical electrophoresisRead more
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WR01001 Each
Catalog number WR0100
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1.325,65
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1.454,00
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Price (EUR)
1.325,65
Online Exclusive
1.454,00
Save 128,35 (9%)
1 Each
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The XCell4 SureLock Midi-Cell is a midi-gel electrophoresis system that allows you to quickly and easily perform simultaneous vertical electrophoresis of 1–4 midi gels. The Midi-Cell is built with tough polycarbonate to boost durability and features a specially designed lid to enhance user safety. The Midi-Cell is designed to dissipate heat effectively and evenly and ensure high-resolution results when using Invitrogen midi gels (8.7 cm x 13.3 cm).

Easy to use—simple assembly and operation for ultimate in ease of use. A single SureLock actuator seals all gels while allowing for zero force insertion and easy positioning for sample loading.
Flexible—run up to four midi-gels at once
Efficient—requires 25% less running buffer per gel than the Criterion Cell (340 mL vs 460 mL)

See all available electrophoresis chamber systems ›

How the system works
The XCell4 SureLock Midi-Cell consists of a lower buffer chamber, two buffer cores, a lid with cables, a displacement dam, and a buffer dam. Electrophoresis of proteins on midi-gels in the XCell4 SureLock Midi-Cell is performed after assembling the buffer core with two midi-gels or one midi-gel and one buffer dam. The buffer core/gel assembly is inserted into the lower buffer chamber using a positive locking mechanism that seals the buffer core/gel assembly firmly into position and ensures leak-free electrophoresis. The buffer core/gel assembly creates separate upper buffer chambers for each gel and a common lower buffer chamber shared by all gels. The lid is placed onto the Midi-Cell, fitting in only one direction.

The Midi-Cell holds gels firmly with a simple gel tension wedge. When the lever on the gel tension wedge is pushed forward into the locked position, an even horizontal force is generated. This seals the gel/buffer core assembly firmly into position in the lower buffer chamber. The positive locking action of the gel tension wedge ensures a trouble-free, leak-free gel run every time.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 Each
Shipping ConditionRoom Temperature
For Use With (Equipment)XCell4 SureLock™ Midi
Gel CompatibilityNovex™ Midi Gels
Gel SizeMidi (8 x 13 cm)
Max. Voltage600 VDC
Product LineXCell SureLock
TypeMidi-Cell Midi-Gel Electrophoresis System
Unit Size1 Each
Contents & Storage
The components of the midi-cell include:
• 1 XCell4 Lower Buffer Chamber
• 1 XCell4 Lid with Cables
• 2 XCell4 Buffer Cores
• 1 XCell4 Buffer Dam
• 1 XCell4 Displacement Dam
• 1 XCell4 Surelock Assembly
• 1 Gel Knife
• 1 Set of 2 Power Supply adapters (Zoom Adapters).

Operating temperature is 4°C to 40°C. The XCell4 Surelock Midi-Cell Runner is guaranteed for 1 year when properly stored.
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Product Information

Frequently asked questions (FAQs)

The XCell4 Displacement Dam is used when fewer than 3 gels are run in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the lower buffer chamber instead of a second buffer core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Recommended maximum load volumes:
12 + 2-well gel: 45 µL in sample well, 15 µL in marker well
20-well gel: 25 µL
26-well gel: 15 µL

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

You will need a Midi Gel Adapter. The Midi Gel Adapter is a self-adhesive, upper buffer chamber used when running the NuPAGE Invitrogen Midi-Gel in a Bio-Rad Criterion Cell. The Midi Gel Adapter may be filled with up to 60 mL of buffer. The Midi Gel Adapters are available packaged with the Midi-Gels (Invitrogen and NuPAGE Invitrogen Midi Gels with Cat. Nos. that end in "A") and also on their own in packages of 10 (Cat. No. WA0999). Running the NuPAGE Midi-Gels in the XCell4 SureLock Gel Apparatus does not require the use of the Midi Gel Adapter.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Multichannel pipette compatibility: 12+2-well (8- and 12-channel pipette-compatible) and 26-well (8- and 12-channel pipette-compatible).
The 12-channel pipettes work with the 26-well gels. You will need to alternate lanes (e.g., aligns with every two lanes). There will be two extra lanes (for MW markers). The 12-channel pipettes work with the 12+2 well gels as well.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend using Invitrogen Power Supply Adapters (Cat. No. ZA10001) to help you connect the leads to your power supply. These adapters are supplied along with the XCell4 SureLock Midi-Cell. If these adapters don't work, you may contact the manufacturer of the power supply to see if other options are available.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Citations & References (2)

Citations & References
Abstract
Serotype IX, a Proposed New Streptococcus agalactiae Serotype.
Authors:Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL,
Journal:J Clin Microbiol
PubMed ID:17634306
'We identified three isolates of Streptococcus agalactiae (group B streptococcus [GBS]), of human origin, which failed to react with antisera against any of the nine known GBS serotypes. Polyclonal rabbit antisera raised against these isolates and standard GBS typing sera were used in capillary precipitation and Ouchterlony tests to compare ... More
Chronic central administration of valproic acid: Increased pro-survival phospho-proteins and growth cone associated proteins with no behavioral pathology.
Authors:Bates RC, Stith BJ, Stevens KE,
Journal:Pharmacol Biochem Behav
PubMed ID:22960225
Valproic acid (VPA) is the most widely prescribed antiepileptic drug due to its ability to treat a broad spectrum of seizure types. However, potential complications of this drug include anticonvulsant polytherapy metabolism, organ toxicity and teratogenicity which limit its use in a variety of epilepsy patients. Direct delivery of VPA ... More
2 total citations

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