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Zip Alexa Fluor™ Rapid Antibody Labeling Kits
Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits allow you to efficiently and quickly label your precious antibody with Alexa Flour dyes to create an antibody conjugate that is ready to use within 15 minutes with 100% recovery.
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| Catalog Number | Quantity | Excitation/Emission | Label or Dye |
|---|---|---|---|
| Z11235 | 3 labelings | 650/665 nm | Alexa Fluor 647 |
| Z11233 | 3 labelings | 490/525 nm | Alexa Fluor 488 |
| Z11234 | 3 labelings | 555/580 nm | Alexa Fluor 555 |
Catalog number Z11235
Price (USD)
375.00
3 labelings
In stock
Quantity:
3 labelings
Excitation/Emission:
650/665 nm
Label or Dye:
Alexa Fluor 647
Price (USD)
375.00
3 labelings
Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits allow you to efficiently and quickly label your precious antibody with Alexa Flour dyes to create an antibody conjugate that is ready to use within 15 minutes with 100% recovery. Each kit contains everything needed for three separate labeling reactions of 100 μg of antibody. Simply add 1 mL water to component A containing Zip buffer, add 100 μg of antibody, incubate for 15 minutes, and your antibody is ready to use. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry (IHC), primary detection, ELISAs, immunocytochemistry (ICC), and indirect FISH.
Create ready-to-use antibody conjugate in just 15 minutes with our Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits. These kits enable you to efficiently label your precious antibody with various Alexa Flour dyes.
Important features of Zip Alexa Fluor Rapid Antibody Labeling kits:
· Speed—antibody conjugates ready to use in 15 min
· Efficiency—100% antibody recovery
· Versatility—kits are useful for a wide range of applications
· Easy-to-use—no chemistry or conjugation experience needed
· Flexibility—choose from Alexa 488, 555, and 647
Each kit contains everything you need to perform three separate labeling reactions of 100 μg of antibody. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry (IHC), primary detection, ELISA, immunocytochemistry (ICC), and indirect FISH.
Benefits of using Zip Alexa Fluor Rapid Antibody Labeling kits:
Specificity and versatility
A primary antibody that is directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Multiple primary antibodies of the same isotype or derived from the same species can be used in the same experiment if they are directly labeled with compatible fluorophores.
Robustness
Compared to traditional dyes, Alexa Fluor dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. When using Alexa Fluor dyes, higher degrees of labeling can usually be achieved without intramolecular quenching.
Customization is possible
Can’t find what you’re looking for in our stocked list? We can prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorFar-Red
Excitation/Emission650/665 nm
Kit ContentsReactive fluorescent label (3 vials), sodium bicarbonate (3 vials)
Label TypeAlexa Fluor
Labeling Scale100 μg
Product LineAlexa Fluor™
Product TypeLabeling Kit
Quantity3 labelings
Chemical ReactivityAmine
Labeling TargetIgG
Label or DyeAlexa Fluor 647
Unit Size3 labelings
Contents & Storage
Store desiccated and protected from light in the freezer (-5°C to +30°C).
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3143947Certificate of AnalysisApr 03, 2025Z11235
3123555Certificate of AnalysisMar 26, 2025Z11234
2935450Certificate of AnalysisJun 14, 2024Z11233
2856071Certificate of AnalysisApr 10, 2024Z11235
2780641Certificate of AnalysisJan 03, 2024Z11234
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Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
No, it is intended for immediate use. If you wish to store your labeled antibody after the reaction, it should undergo a post-reaction clean-up using either size exclusion resin (such as Bio-Rad BioGel P-30) or Dye Removal Columns (Cat No. 22858).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
This is because we have optimized the amount of dye to label 100 µg of antibody per reaction and after the incubation time of 15 mins, most of the reactive dye is either bound or unreactive due to spontaneous hydrolysis, leaving little or no reactive dye to label your sample.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes, the BSA and any component with a primary amine (such as the Tris buffer) would react with the amine-reactive Alexa Fluor dyes used in these labeling kits, resulting in less optimal labeling of the antibody. For small volume purification of the antibody, you may use either Nab Protein A/G Spin Kit, 0.2 mL (Cat No. 89950), Protein A/G Magnetic Agarose Beads (Cat No. 78609), or Melon Gel IgG Spin Purification Kit (Cat No. 45206).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The 1 mg/mL concentration of the antibody (100 µg of antibody in a 100 µL volume) allows for optimal reaction kinetics. A more dilute solution of antibody would exhibit suboptimal labeling due to a greater amount of reactive dye lost to spontaneous hydrolysis; the reactive group on the dyes is labile in aqueous solutions.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Each kit contains 3 vials of solid sodium bicarbonate (Zip buffer reagent, Component A), 3 vials of the Alexa Fluor reactive dye (Component B), and a Quick Reference Card. Sufficient reagents are provided to label 100 µg of antibody per reaction (100 µL of a 1 mg/mL antibody solution), 3 reactions per kit.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.