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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • RNF3 Antibodies

            NeoBiotechnologies

            PCGF3 Monoclonal Antibody (PCRP-PCGF3-1D5)

            View all (10) RNF3 antibodies
            Datasheet
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            Cite PCGF3 Monoclonal Antibody (PCRP-PCGF3-1D5)

            • Antibody Testing Data (2)
            Application
            PCGF3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            Group 53 Created with Sketch.

            FIGURE: 1 / 2

            PCGF3 Antibody (10336-MSM2-P1ABX) in IHC (P)

            Formalin-fixed, paraffin-embedded human prostate stained with PCGF3 Mouse Monoclonal Antibody (PCRP-PCGF3-1D5) HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.
            View Product

            PCGF3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            PCGF3 Antibody in Flow Cytometry (Flow)

            Product Details

            10336-MSM2-P1ABX

            Applications
            Tested Dilution
            Publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1-2 µg/mL
            -

            Immunocytochemistry (ICC/IF)

            2-4 µg/mL
            -

            Flow Cytometry (Flow)

            1-2 µg/mL
            -

            Peptide Array (Array)

            Assay-dependent
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Mouse / IgG2b

            Class

            Monoclonal

            Type

            Antibody

            Clone

            PCRP-PCGF3-1D5

            Immunogen

            Recombinant full-length human PCGF3 protein
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Protein A/G

            Storage buffer

            PBS

            Contains

            no preservative

            Storage conditions

            -20°C or -80°C if preferred

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Positive Control: HeLa or MCF-7 cells. Human brain testis or smooth muscle.

            Cellular Location: Nucleus.

            Target Information

            Phosphorylase kinase is a hexadecameric enzyme that is comprised of four copies of four subunits that are encoded by four separate genes: PHKA, PHKB, PHKG, and PH kDa. This serine/threonine specific kinase converts glycogen phosphorylase b to glycogen phosphorylase a, resulting in the release of glucose-1-phophate from glycogen. PHKB (Phosphorylase b kinase regulatory subunit beta) is a 1093 amino acid subunit of phosphorylase kinase that, along with PHKA, has regulatory functions controlled by phosphorylation. Defects in the gene encoding PHKB are the cause of glycogen storage disease type 9B, which is also known as phosphorylase kinase deficiency of liver and muscle. This disease is characterized by a mild phenotype of hepatomegaly with only slightly elevated transaminase and plasma lipids, no clinical muscle involvement, and generally is correlated with a gradual improvement with increasing age. There are four isoforms of PHKB that are produced as a result of alternative splicing events.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

            Have you cited this product in a publication?
            Let us know so we can reference it here.
            Cite this product

            Bioinformatics

            Protein Aliases: D6S113E; DADB-17J1.3; DKFZp686D20235; DKFZp686N0336; FLJ31942; FLJ36550; FLJ43813; FSH; FSRG1; KIAA9001; MGC129615; MGC40413; NAT; Polycomb group RING finger protein 3; ring finger protein 3; RING finger protein 3A; RING3

            View more View less

            Gene Aliases: DONG1; PCGF3; RNF3; RNF3A

            View more View less

            UniProt ID: (Human) Q3KNV8

            View more View less

            Entrez Gene ID: (Human) 10336

            View more View less

            Function(s)

            protein binding zinc ion binding histone modifying enzyme

            Process(es)

            transcription, DNA-templated regulation of transcription, DNA-templated
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
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            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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