Inhibidor de la ribonucleasa recombinante RNaseOUT™
Inhibidor de la ribonucleasa recombinante RNaseOUT™
Invitrogen™

Inhibidor de la ribonucleasa recombinante RNaseOUT™

El inhibidor de ribonucleasa recombinante RNaseOUT™ es un potente inhibidor no competitivo de ribonucleasas de tipo pancreático como ARNasa A.Más información
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Número de catálogoCantidad
107770195000 units
Número de catálogo 10777019
Precio (USD)
-
Cantidad:
5000 units
Pedido a granel o personalizado
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El inhibidor de ribonucleasa recombinante RNaseOUT™ es un potente inhibidor no competitivo de ribonucleasas de tipo pancreático como ARNasa A. Se utiliza para evitar la degradación del ARN en una serie de aplicaciones. El inhibidor de ribonucleasa recombinante RNaseOUT™ es una proteína acídica con un peso molecular de ∼52 kDa. RNaseOUT™ inhibe la ARNasa A, RNasa B y RNasa C.

Aplicaciones
Síntesis de la ADNc, RT-PCR y transcripción y translación in vitro

Fuente
Purificado por cromatografía de afinidad a partir de la expresión de un gen porcino clonado de E. coli

Pruebas de rendimiento y calidad
Pureza SDS-PAGE, ensayos de endodesoxiribonucleasa, concentración de proteínas, actividad específica, rendimiento evaluado mediante RT-PCR

Definición de la unidad
Una unidad inhibe 5 ng de ARNasa A en un 50 % mediante el uso de citidina 2´, 3´ monofosfato cíclico (cCMP) como sustrato

Condiciones de reacción de la unidad
100 mM de tris-acetato (pH 6,5), 1 mM de EDTA, 0,2 mM de cCMP, 2 µg de ARNasa A en 1 ml de 0 a 10 minutos a 25 °C
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración100 mm
Línea de productosRNaseOUT™
Tiempo de purificación10 min
Cantidad5000 units
Condiciones de envíoHielo seco
Unit SizeEach
Contenido y almacenamiento
Almacenar a -20 °C. Evite exponer el producto a cambios frecuentes de temperatura. El inhibidores de ribonucleasa RNaseOUT™ requiere 1 mm de DTT para mantener la actividad.

El producto tiene una garantía de 6 meses a partir de la fecha de compra, a menos que se indique lo contrario en la documentación del producto.
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Preguntas frecuentes

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

What does RNaseOUT Recombinant RNase Inhibitor do?

The inhibitor acts as a safeguard against degradation of target RNA due to ribonuclease contamination of the RNA preparation.

What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?

RNaseOUT Recombinant Ribonuclease Inhibitor is provided at a concentration of 40 U/µL.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?

RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019) unit reaction conditions are defined at 25 degrees C. The upper temperature limit for full functionality is 40 degrees C. The enzyme half-life is decreased as temperatures increase above 40 degrees C. There is some residual activity up to 50-55 degrees C but heating at 65 degrees C will inactivate the enzyme. As RNaseOUT can be used in various applications like cDNA synthesis, RT-PCR, and in vitro transcription, the recommendation is to follow the temperature settings required for the respective method.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I am having trouble performing PCR amplification after DNA isolation with RNAlater-treated samples. Does RNAlater have an impact on downstream PCR applications?

RNAlater should not impact downstream PCR amplification as long as the sample has been properly cleaned before proceeding with DNA isolation, as stated in the RNAlater Stabilization Solution manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/7020M.pdf) on page 7.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

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