Tampón de disociación celular, sin enzimas, PBS

Gibco™

Tampón de disociación celular, sin enzimas, PBS

El tampón de disociación celular Gibco™ es una solución isotónica, sin enzimas y filtrada por membrana de sales, agentes quelantesMás información

Número de catálogo	Cantidad
13151014	100 mL

Número de catálogo 13151014

Precio (USD)

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Cantidad:

100 mL

El tampón de disociación celular Gibco™ es una solución isotónica, sin enzimas y filtrada por membrana de sales, agentes quelantes y agentes de recuento celular en una solución salina tamponada con fosfato (PBS) sin calcio ni magnesio. El tampón de disociación celular Gibco™ es adecuado para la disasociación de células de mamíferos para estudios como unión de ligandos, citometría de flujo e inmunohistoquímica, que requieren proteínas de superficie celular intactas. El tampón de disociación celular Gibco™ funciona bien con líneas celulares ligeramente adherentes como HeLa y NIH 3T3, pero no se recomienda para el pasaje rutinario de células fuertemente adherentes. El protocolo de disociación está disponible en nuestra biblioteca de referencia técnica.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Línea de célulasHeLa, NIH 3T3

QuelantesEDTA

Cantidad100 mL

NotasTemperatura ambiente estable

Duración de almacenamiento24 meses

Condiciones de envíoTemperatura ambiente

ClasificaciónLibre de material de origen animal

Tipo de productoReactivo para disociación de cultivo celular

EsterilidadEstéril con filtro

Con aditivosEDTA

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De 15 °C a 30 °C
Condiciones de envío: Temperatura ambiente
Vida útil: 24 meses a partir de la fecha de fabricación

Preguntas frecuentes

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

Below are QC test specifications for pH and osmolality for this product:

- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

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Tampón de disociación celular, sin enzimas, PBS

Preguntas frecuentes

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

What reagents do you offer for cell dissociation, and what are the main differences between them?

Documentos y descargas

Certificados

Hojas de datos de seguridad