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Thermo Scientific™
Pierce™ Protein Coated Plates
Thermo Scientific™ Pierce Protein A, G, A/G and L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.
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| Número de catálogo | Tratamiento de superficie | Descripción |
|---|---|---|
| 15138 | Proteína A/G | Transparente, tira de 8 pocillos |
| 15132 | Proteína A | Transparente, tira de 8 pocillos |
| 15130 | Proteína A | Transparente, 96 pocillos |
| 15155 | Proteína A | Negro, 96 pocillos |
| 15154 | Proteína A | Blanco, 96 pocillos |
| 15133 | Proteína G | Transparente, tira de 8 pocillos |
| 15131 | Proteína G | Transparente, 96 pocillos |
| 15157 | Proteína G | Negro, 96 pocillos |
| 15156 | Proteína G | Blanco, 96 pocillos |
| 15190 | Proteína L | Transparente, 96 pocillos |
Número de catálogo 15138
Precio (USD)/ Each
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Tratamiento de superficie:
Proteína A/G
Descripción:
Transparente, tira de 8 pocillos
These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L). They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.
Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.
Highlights
• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)
Protein A/G Coated Plates
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH
Protein A Coated Plates
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation
Protein G Coated Plates
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation
Protein L Coated Plates
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Propiedad de uniónCapacidad estándar, afinidad
DescripciónTransparente, tira de 8 pocillos
Método de detecciónColorimétrico
Línea de productosPierce™
ColorTransparente
Tipo de productoPlaca de detección, inmunoensayo, ELISA
MaterialPoliestireno
N.° de pocillos96
Tratamiento de superficieProteína A/G
N.° por paquete5 placas
Bloqueo de placasTampón SuperBlock™
Cantidad5 plates
Unit SizeEach
Contenido y almacenamiento
Nota: Las placas se activan a 100 µl y se suministran prebloqueadas con tampón bloqueante Thermo Scientific SuperBlock. Utilice estas placas solo para ensayos de un solo anticuerpo. En ensayos de varios anticuerpos, como los ensayos ELISA de tipo sándwich, el primer anticuerpo no puede bloquear todos los puntos de unión y los anticuerpos posteriores se unen a la placa, lo que da lugar a falsos positivos.
Tras su recepción, almacene las placas a 4 °C en bolsas sin abrir. Coloque las placas abiertas y no utilizadas en una bolsa resellable y almacénelas desecadas a 4 °C. Las placas se envían a temperatura ambiente.
Tras su recepción, almacene las placas a 4 °C en bolsas sin abrir. Coloque las placas abiertas y no utilizadas en una bolsa resellable y almacénelas desecadas a 4 °C. Las placas se envían a temperatura ambiente.