Thermo Scientific™

Pierce™ Bradford Plus Protein Assay Kits

Número de catálogo: 23236
Thermo Scientific™

Pierce™ Bradford Plus Protein Assay Kits

Número de catálogo: 23236
The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent used to quickly measure total protein concentration compared to a protein standard. It provides increased linearity and half the protein-to-protein variability of other commercial Bradford assay formulations.
Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
 
Número de catálogo
23236
Formato
950 mL
Descripción
Kit de ensayo de proteínas Pierce Coomassie Plus (Bradford)
Contenido del kit
10 x 1 mL glass ampules
Precio (USD)
Disponibilidad
***
Especificaciones completas
Cantidad
-
ConcentraciónBSA: 2 mg/mL
DescripciónKit de ensayo de proteínas Pierce Coomassie Plus (Bradford)
ensayoEnsayo de Bradford
Método de detecciónColorimétrico
Línea de productosPierce™
Tipo de productoEnsayo de cuantificación de proteínas
EspecificidadSin especificidad
Suficiente para630 ensayos en tubo o 3160 ensayos en microplaca
Para utilizar con (aplicación)Detección basada en soluciones, absorbancia
Para utilizar con (equipo)Espectrofotómetro, lector de microplacas
Contenido del kit10 x 1 mL glass ampules
Cantidad950 mL
Unit Size950 mL
Número de catálogo
Formato
950 mL
Descripción
Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards
Contenido del kit
2 x 1 mL 8-channel tubestrip, prediluted
Precio (USD)
Precio: 290,00
Su precio:
Disponibilidad
***
Especificaciones completas
Cantidad
ConcentraciónBSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL
DescripciónPierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards
ensayoBradford Assay
Método de detecciónColorimetric
Línea de productosDilution-Free™
Tipo de productoProtein Quantitation Assay
EspecificidadNot Target-specific
Suficiente para630 Tube Assays or 3160 Microplate Assays
Para utilizar con (aplicación)Solution-based Detection, Absorbance
Para utilizar con (equipo)Spectrophotometer, Microplate Reader
Contenido del kit2 x 1 mL 8-channel tubestrip, prediluted
Cantidad950 mL
Unit Size950 mL
Mostrando 2 de 2
Número de catálogoEspecificacionesFormatoDescripciónContenido del kitPrecio (USD)DisponibilidadCantidad
23236Especificaciones completas
950 mLKit de ensayo de proteínas Pierce Coomassie Plus (Bradford)10 x 1 mL glass ampulesContáctenos ›***-
ConcentraciónBSA: 2 mg/mL
DescripciónKit de ensayo de proteínas Pierce Coomassie Plus (Bradford)
ensayoEnsayo de Bradford
Método de detecciónColorimétrico
Línea de productosPierce™
Tipo de productoEnsayo de cuantificación de proteínas
EspecificidadSin especificidad
Suficiente para630 ensayos en tubo o 3160 ensayos en microplaca
Para utilizar con (aplicación)Detección basada en soluciones, absorbancia
Para utilizar con (equipo)Espectrofotómetro, lector de microplacas
Contenido del kit10 x 1 mL glass ampules
Cantidad950 mL
Unit Size950 mL
A55866Especificaciones completas
950 mLPierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards2 x 1 mL 8-channel tubestrip, prediluted
Precio: 290,00
Su precio:
***
ConcentraciónBSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL
DescripciónPierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards
ensayoBradford Assay
Método de detecciónColorimetric
Línea de productosDilution-Free™
Tipo de productoProtein Quantitation Assay
EspecificidadNot Target-specific
Suficiente para630 Tube Assays or 3160 Microplate Assays
Para utilizar con (aplicación)Solution-based Detection, Absorbance
Para utilizar con (equipo)Spectrophotometer, Microplate Reader
Contenido del kit2 x 1 mL 8-channel tubestrip, prediluted
Cantidad950 mL
Unit Size950 mL
Mostrando 2 de 2

The Pierce Bradford Plus Protein Assay Kit is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent used to quickly measure total protein concentration compared to a protein standard. It provides increased linearity and half the protein-to-protein variability of other commercial Bradford assay formulations.

Kit options include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.

Features of the Pierce Bradford Plus Protein Assay Kit include:

  • Colorimetric—measure with a standard spectrophotometer or plate reader at 595 nm
  • Easy to use—single reagent; no working reagent preparation required
  • Fast—almost immediate color development; add, mix, and read results
  • Wide assay range—detects protein concentration in the range 1 to 1500 μg/mL
  • Linear—improved linearity and response uniformity compared to traditional Bradford formulations
  • Flexible—microplate and cuvette protocols provided and adaptable to several target working ranges
  • Serial dilution–free—select kit options include Dilution-Free BSA Protein Standards that eliminate the need to perform serial dilutions when generating a standard curve

The assay is performed at room temperature. Simply add the sample to the tube or well containing reagent, and the resultant blue color is measured at 595 nm following a short room-temperature incubation. The protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal-chelating agents encountered in protein samples. The assay can be performed in either test tube or microplate format.

How the Bradford Plus Assay detects protein

Use of Coomassie G-250 dye in a colorimetric reagent for the detection and quantitation of total protein was first described by Dr. Marion Bradford in 1976. In the acidic environment of the reagent, protein binds to the Coomassie dye. This results in a spectral shift from the reddish/brown form of the dye (absorbance maximum at 465 nm) to the blue form of the dye (absorbance maximum at 610 nm). The difference between the two forms of the dye is greatest at 595 nm, so that is the optimal wavelength to measure the blue color from the Coomassie dye-protein complex. If desired, the blue color can be measured at any wavelength between 575 nm and 615 nm. At the two extremes (575 nm and 615 nm) there is a loss of about 10% in the measured amount of color (absorbance) compared to that obtained at 595 nm.

Development of color in Coomassie dye-based (Bradford) protein assays has been associated with the presence of certain basic amino acids (primarily arginine, lysine, and histidine) in the protein. Van der Waals forces and hydrophobic interactions also participate in the binding of the dye by protein. The number of Coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Free amino acids, peptides, and low molecular weight proteins do not produce color with Coomassie dye reagents. In general, the mass of a peptide or protein must be at least 3000 Daltons to be assayed with this reagent.

For Research Use Only.
For Research Use Only. Not for use in diagnostic procedures.

Contenido y almacenamiento

Suficiente para: 630 ensayos de tubos o 3160 ensayos de microplacas
• Reactivo de ensayo Pierce Coomassie Plus, 950 ml
• Ampollas de patrón de albúmina, 2 mg/ml, 10 x 1 ml

Almacene el reactivo de ensayo Coomassie Plus a 4 °C. Almacene las ampollas de patrón de albúmina sin abrir a temperatura ambiente.

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