ReproSeq™ PGS 316 View Kit, with chips

The ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The ReproSeq™ PGS 316 View Kit, with chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 120 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
• Ion 316™ Chip Kit v2 BC (4488149)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System

Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.