AccelerOme™ Label-Free MS Sample Prep Kit

The Thermo Scientific AccelerOme Label-Free MS Sample Prep Kit provides ready-to-go reagents for efficient and robust proteomics mass spectrometry (MS) sample preparation for use with the AccelerOme Automated Sample Prep System.

Features of the Label-Free MS Sample Prep Kit include:
• Standardized—EasyPep kit chemistry designed for use with AccelerOme Automated Sample Prep System
• Convenient—complete set of ready-to-use proteomic sample prep and clean-up reagents for easy loading onto instrument
• Robust—reagents combined with AccelerOme ecosystem provide consistent, high-quality results with technical replicates with <5% CV
• Flexible—reagents verified on system using cells, plasma, proteins, and tissue samples for 1-–100 μg samples
• Time-saving—instrumentation and reagents perform the work enabling time away from tedious hands-on sample preparation

The AccelerOme Label-Free MS Sample Prep Kit contains pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean up cartridges for the processing of samples from protein lysis and digestion through peptide clean-up. The single-use components are conveniently provided in labeled trays for direct placement on the AccelerOme system. A peptide retention time calibration standard is also included with the kit (shipped separately) that can be spiked into the sample before LC-MS analysis to assess system performance. This kit provides a complete reagent solution for automated sample preparation using proteins, plasma, cells, and tissue to produce high quality, MS-ready peptides.

The AccelerOme reagent portfolio is an integral part of the AccelerOme Automated system solution to provide robust proteomic MS sample preparation. The instrument hardware is built on a proven liquid handling format for reproducible sample processing and optimized on-line μSPE sample clean-up for excellent peptide recovery. Peptide quantitation post-cleanup is achieved through accurate UV spectrophotometric analysis to determine peptide concentrations prior to LC-MS analysis. The complete system (kits + reagents + software) was designed to provide high quality peptides while improving productivity with an easy-to-use automated system.