Kit de ensayo de proteasa EnzChek™, fluorescencia verde, 100-1000 ensayos
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Kit de ensayo de proteasa EnzChek™, fluorescencia verde, 100-1000 ensayos

El kit de ensayo de proteasas EnzChek™, fluorescencia verde, es un ensayo rápido, simple y directo basado en la fluorescenciaMás información
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Número de catálogoCantidad
E66381 kit
Número de catálogo E6638
Precio (USD)
-
Cantidad:
1 kit
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El kit de ensayo de proteasas EnzChek™, fluorescencia verde, es un ensayo rápido, simple y directo basado en la fluorescencia para la detección de metalo-, serina, ácido, y sulfhidrilo proteasas. El aumento de la fluorescencia que lo acompaña, que puede medirse con un lector de microplacas de fluorescencia, es proporcional a la actividad de la proteasa.

Consulte nuestra línea completa de ensayos de microplaca de fluorescencia.

• El mecanismo generalizado permite medir una variedad de proteasas
• Puede utilizarse para medir continuamente la cinética de la proteasa
• La excitación/emisión de 505/513 nm permite el uso de ajustes de filtro estándar de fluoresceína (FITC)

Este kit de ensayo de proteasas EnzChek™ contiene un derivado de la caseína que ha sido ampliamente marcado con el colorante BODIPY™ FL, insensible al pH y verde fluorescente, lo que provoca un apagado del colorante fluorescente. La hidrólisis catalizada por la proteasa libera los péptidos altamente fluorescentes etiquetados con el colorante BODIPY™ FL, lo que permite la detección cuantitativa de la actividad de la proteasa en la solución. El colorante verde fluorescente BODIPY™ FL tiene espectros de excitación y emisión similares a los de la fluoresceína.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónIntensidad de la fluorescencia
Cantidad1 kit
Categoría de investigaciónDiferenciación
Condiciones de envíoTemperatura ambiente
Propiedades de sustratoSustrato basado en proteínas
Tipo de sustratoSustrato de proteasa
Enzima dianaProteasas
Para utilizar con (aplicación)Ensayo de proteasa
Para utilizar con (equipo)Lector de microplacas de fluorescencia
Línea de productosEnzChek™
Tipo de productoEnsayo de proteasa
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
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Preguntas frecuentes

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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N.º de loteCertificate TypeDateCatalog Number(s)
3101945Certificate of Analysis10 dic 2024E6638
2626261Certificate of Analysis09 jul 2023E6638
2497537Certificate of Analysis03 jul 2022E6638
2395139Certificate of Analysis02 sept 2021E6638
2398034Certificate of Analysis07 ago 2021E6638
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Product Information

Citations & References (19)

Citations & References
Abstract
Authors:
Journal:
PubMed ID:10748021
Effective antiprotease-antibiotic treatment of experimental anthrax.
Authors:Popov SG, Popova TG, Hopkins S, Weinstein RS, MacAfee R, Fryxell KJ, Chandhoke V, Bailey C, Alibek K
Journal:BMC Infect Dis
PubMed ID:15819985
'BACKGROUND: Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus anthracis. It causes severe damage, including multiple hemorrhagic lesions, to host tissues and organs. It is widely believed that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of ... More
Enzymatic microreactor-on-a-chip: protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devices.
Authors:Peterson DS, Rohr T, Svec F, Fréchet JM
Journal:Anal Chem
PubMed ID:12199578
'Enzymatic microreactors have been prepared in capillaries and on microfluidic chips by immobilizing trypsin on porous polymer monoliths consisting of 2-vinyl-4,4-dimethylazlactone, ethylene dimethacrylate, and acrylamide or 2-hydroxyethyl methacrylate. The azlactone functionalities react readily with amine and thiol groups of the enzyme to form stable covalent bonds. The optimized porous properties ... More
Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.
Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP
Journal:Anal Biochem
PubMed ID:9299009
'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More
Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
Authors:Nolkrantz K, Farre C, Hurtig KJ, Rylander P, Orwar O
Journal:Anal Chem
PubMed ID:12199607
'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased ... More
19 total citations

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