The following components should be stored at –20°C:
- Calf Intestine Alkaline Phosphatase
- 10X CIP Buffer
- Tobacco Acid Pyrophosphatase
- 10X TAP Buffer, T4 RNA Ligase
- 10X T4 RNA Ligase Buffer
- 5' RACE Adapter
- 3' RACE Adapter
- 5' RACE Outer Primer
- 5' RACE Inner Primer
- 3' RACE Outer Primer
- 3' RACE Inner Primer
- Ammonium Acetate Stop Solution
- M-MLV Reverse Transcriptase
- dNTP Mix
- 10X RT Buffer
- 10X PCR Buffer
- Random Decamers
- RNase Inhibitor
- Mouse Thymus RNA
- 5' RACE Outer Control Primer
- 5' RACE Inner Control Primer
- 5' PCR Control Primer
- 3' RACE Control Primer
Nuclease-free water may be stored at any temperature.