Lumio™ Technology is designed for simple fluorescent detection of recombinant protein. The Lumio™ tag is a small (six amino acid) sequence that binds a fluorescent substrate, allowing you to visually detect protein localization in mammalian cells. The Mammalian Lumio™ Gateway™ vectors (Figure 1) offer the following features:
• Lumio™ tag for accurate in vitro and in vivo protein detection • CMV promoter for high-level constitutive expression • tR sites for efficient recombination with Gateway™ entry clones • Blasticidin resistance gene for fast, efficient selection
Use Mammalian Lumio™ Gateway™ vectors for reliable and consistent protein expression, detection, and localization in mammalian cells (Figure 2). Vectors are available for generating protein with an N- and C-terminal Lumio™ fusion tag. In addition, each Mammalian Lumio™ Gateway™ Kit includes a Lumio™ Green, Red, or Dual In-Cell Detection Kit. The Dual In-Cell Detection Kit is ideal for doing two color “pulse-chase” experiments to examine protein localization over time.
Especificações
Detection Method
Fluorescent
For Use With (Equipment)
Fluorescence Microscope, Electron Microscope
Product Type
In-Cell Detection Kit
Detection Location
In-Cell Detection
Protein Tag
Lumio
Label or Dye
Lumio™ Green
Product Line
Lumio™
Color
Green
Conteúdo e armazenamento
The Lumio In-Cell Detection kits include 40 μL of Lumio Green or Red fluorescent reagent as a 2 mM stock solution in DMSO. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Figuras
Documentos e downloads
Certificados
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