The TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix. Gene-specific, ready to use TaqMan™ Gene Expression Assays and Universal Master Mix provide you with a fast, simple way for measuring changes in gene expression.
• Simple procedure gives you quick results • Hot-Start DNA Polymerase enzyme system minimizes nonspecific product amplification for reliable results
Ready-to-Use Gene Expression Solution The TaqMan™ Reagents Starter Kit contains a sample of TaqMan™ Universal PCR Master Mix, and TaqMan™ Gene-Expression Assay (Eukaryotic 18S rRNA), plus a sample of human Raji cDNA - everything you need for 18S gene-expression analysis. Simply combine these reagents according to the instructions to generate reproducible and quantitative gene-expression data.
A Complete, Integrated Gene-Expression System Eliminate time-consuming master mix formulation and assay optimization with the TaqMan™ Gene-Expression Assays and the TaqMan™ Universal PCR Master Mix, by using universal cycling conditions and pre-optimized reagents.
Used With 7500 Real-Time PCR System with Dell™ Notebook, 7500 Real-Time PCR System with Dell™Tower, 7900HT Fast Real-Time PCR System with 384-Well Block Module, 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module, 7900HT System Automation Accessory Upgrade, 7900HT System Standard 384-Well Block Upgrade Kit, 7900HT System Standard 96-Well Block Upgrade Kit, ABI PRISM™ 7000 Sequence Detection System (Notebook).
For Research Use Only. Not for use in diagnostics procedures.
Especificações
Detection Method
Primer-probe
PCR Method
qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
For Use With (Equipment)
7000 System, 7500 System, AB 7900 HT 384-Well System
Passive Reference Dye
ROX (Pre-mixed)
Product Line
TaqMan™
Product Type
PCR Reagents
Shipping Condition
Dry Ice
Target
18S rRNA
Target Specificity
Target-Specific
For Use With (Application)
Gene Expression
Hot Start
Built-In Hot Start
Sample Type
DNA (Genomic), cDNA
Conteúdo e armazenamento
This kit contains TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG, Eukaryotic 18S rRNA TaqMan™ Assay (with two unlabeled PCR primers and a labeled TaqMan™ probe), plus a sample of human Raji cDNA.
Store the PCR mix at 2° to 8°C. Store the rest of the kit contents at -15° to -25°C.
Figuras
Documentos e downloads
Certificados
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