The Oncomine IGH FR1-J Assay is a targeted next-generation sequencing (NGS) assay for detection of somatic hypermutation (SHM) in the variable region of the immunoglobulin heavy chain of B-cell receptors. SHM increases the affinity of the B-cell receptor for antigen, post-VDJ recombination, and is frequently a region of interest in CLL research. Primers have been specifically designed to target framework 1 and joining gene regions of the immunoglobulin heavy chain, resulting in an amplicon ∼300 bp in length. When combined with Ion Reporter Software, you can easily analyze your data and create reports within a short period of time.
Key features of the Oncomine IGH FR1-J Assay: • Sequencing of FR1-J region of IGH chain • Detects SHM in CLL samples with high degree of sensitivity • Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots
The kit provides reagents for library construction, including dNTPs. Ion AmpliSeq libraries can be generated from DNA extracted from fresh/frozen tissue, bone marrow, whole blood, peripheral blood leukocytes, PBMCs, and sorted B cells with as little as 50 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 System with the Ion 530 Chip to enable up to eight samples per run.
Especificações
Control Template
dNTP mix
Control Type
Ion AmpliSeq Library Plus Kit
Sample Type
DNA
Sequencing Type
Genome and DNA Sequencing
For Use With (Equipment)
GeneStudio
For Use With (Application)
Sequencing
Libraries
targeted sequencing
No. of Reactions
24
Shipping Condition
Dry Ice
Conteúdo e armazenamento
• Oncomine IGH FR1-J Panel • dNTP mix • Ion AmpliSeq Library Plus Kit All components stored at -10 to -30°
Figuras
Documentos e downloads
Certificados
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