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Invitrogen™
RNAqueous™-4PCR Total RNA Isolation Kit
Número do catálogo: AM1914
Invitrogen™
RNAqueous™-4PCR Total RNA Isolation Kit
Número do catálogo: AM1914
Número do catálogo
AM1914
Tamanho da unidade
30 preps
Preço (BRL)
Preço 1.833,12
Seu Preço
Disponibilidade
***
Quantidade
| Número do catálogo | Tamanho da unidade | Preço (BRL) | Disponibilidade | Quantidade | |
|---|---|---|---|---|---|
| AM1914 | 30 preps | Preço 1.833,12 Seu Preço | *** |
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The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (∼100 cells to ∼1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:
• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous™-4PCR Kit
The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous™-4PCR Kit
The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
Especificações
Isolation Technology
Silica Spin Column
Purification Time
10 min. (After sample homogenization)
Elution Volume
50–120 μL
Sample Type
Tissue, Cells
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
30 Preps
Shipping Condition
Box 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
Starting Material Amount
Tissue: ≤75 mg
Cells: ≤107
Cells: ≤107
Yield
≤10 μg per 1 mg tissue
≤1 μg per 1 x 105 cells
≤1 μg per 1 x 105 cells
Conteúdo e armazenamento
• 600 μL 10X DNase 1 Buffer; store at -20°C
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature
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