Gibco™ Hepatocyte Thaw Medium is a ready-to-use medium specifically designed for use with thawed cryopreserved hepatocytes to enhance their viability and overall quality. This medium is provided in a 50 mL conical tube for ease of use.

Features of Hepatocyte Thaw Medium:

• Easy to use—simply warm up to 37°C and add thawed hepatocytes
• Recover cryopreserved hepatocytes with a single centrifugation
• Designed for use with Gibco™ human and animal cryopreserved hepatocytes for optimal results
• Provides high quality hepatocytes suitable for many in vitro assays, including induction, metabolic stability, and transporter assessments

Hepatocyte Thaw Medium is easy to use. Simply warm the tube of media to 37°C prior to thawing the cryopreserved hepatocytes. Once the medium is warm, thaw the cryopreserved hepatocytes per the Gibco™ protocol. Once the hepatocytes are thawed, pour the cells directly into the pre-warmed tube of Hepatocyte Thaw Medium and centrifuge. Remove the supernatant and proceed with the next steps of your experiment. There is no need to centrifuge the hepatocytes a second time.