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Número do catálogo
V803001
Tamanho da unidade
1 kit
Preço (BRL)
Preço 6.325,00
Seu Preço
Disponibilidade
***
Quantidade
| Número do catálogo | Tamanho da unidade | Preço (BRL) | Disponibilidade | Quantidade | |
|---|---|---|---|---|---|
| V803001 | 1 kit | Preço 6.325,00 Seu Preço | *** |
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pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
Especificações
Promoter
OplE2
Product Type
Insect Cell Expression Vector
Protein Tag Position (to your gene)
C-terminal
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Vector
pMIB
Product Line
InsectSelect™
Conteúdo e armazenamento
The pMIB/V5-His Vector Kit contains 20 μg each of supercoiled, lyophilized pMIB/V5-His A, B, and C vectors, pMIB/V5-His/CAT control vector, and 2 μg each of the OpIE2 Forward and Reverse Sequencing primers. Store at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
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