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Invitrogen™
GLOBINclear™ Kit, mouse/rat, for globin mRNA depletion
The Ambion™ GLOBINclear™-Mouse/Rat Kit employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA Read more
Catalog number AM1981
Price (CLP)/ 20 preps
Price:650.811
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Price (CLP)/ 20 preps
Price:650.811
GLOBINclear™ Kit, mouse/rat, for globin mRNA depletion
Catalog numberAM1981
Price (CLP)/ 20 preps
Price:650.811
-
The Ambion™ GLOBINclear™-Mouse/Rat Kit employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood. The kit includes sufficient reagents for 20 reactions.
• Remove >95% of unwanted globin mRNA from whole blood total RNA
• Detect up to 50% more genes—analyze previously undetected genes
• Achieve greater representation of the genome, low 3'/5' ratios
• Avoid RNase H treatments that can degrade mRNA and alter expression profiles
It is widely known that expression array data generated from whole blood total RNA samples have reduced detection sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a dominant band in the 600–700 base region. This band represents globin mRNA, which is expressed at high levels in red blood cells (RBCs) and reticulocytes (RBC precursors). Up to 70% of the mRNA (by mass) in whole blood total RNA are globin transcripts. Array analysis using total RNA containing a high percentage of globin mRNA has been shown to:
• Decrease present calls
• Decrease call concordance
• Increase signal variation
The GLOBINclear™ kit employs a novel hybridization technology that takes advantage of the strength of biotin/streptavidin binding, the specificity of nucleic acid hybridization, and the convenience of magnetic separations. There are no harsh RNase H enzymatic treatments that may compromise the expression profile by degrading mRNAs other than globin mRNA. The resulting mRNA is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR. The enrichment delivers a significant increase in Percent Present calls, decreased sample-to-sample variability, and low 3'/5' ratios.
Accessory Product:
The kit requires the use of a magnetic stand. The 6 Tube Magnetic Stand (SKU# AM10055) can hold six separate 1.5 mL conical microcentrifuge tubes or 2 mL round-bottomed microtubes with a diameter of 8–10 mm.
• Remove >95% of unwanted globin mRNA from whole blood total RNA
• Detect up to 50% more genes—analyze previously undetected genes
• Achieve greater representation of the genome, low 3'/5' ratios
• Avoid RNase H treatments that can degrade mRNA and alter expression profiles
It is widely known that expression array data generated from whole blood total RNA samples have reduced detection sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a dominant band in the 600–700 base region. This band represents globin mRNA, which is expressed at high levels in red blood cells (RBCs) and reticulocytes (RBC precursors). Up to 70% of the mRNA (by mass) in whole blood total RNA are globin transcripts. Array analysis using total RNA containing a high percentage of globin mRNA has been shown to:
• Decrease present calls
• Decrease call concordance
• Increase signal variation
The GLOBINclear™ kit employs a novel hybridization technology that takes advantage of the strength of biotin/streptavidin binding, the specificity of nucleic acid hybridization, and the convenience of magnetic separations. There are no harsh RNase H enzymatic treatments that may compromise the expression profile by degrading mRNAs other than globin mRNA. The resulting mRNA is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR. The enrichment delivers a significant increase in Percent Present calls, decreased sample-to-sample variability, and low 3'/5' ratios.
Accessory Product:
The kit requires the use of a magnetic stand. The 6 Tube Magnetic Stand (SKU# AM10055) can hold six separate 1.5 mL conical microcentrifuge tubes or 2 mL round-bottomed microtubes with a diameter of 8–10 mm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeMouse/Rat Kit
Final Product TypeTotal RNA (Globin mRNA-Depleted)
High-throughput CompatibilityHigh-throughput Compatible
Sample TypeTotal RNA (Whole Blood)
SpeciesMouse, Rat
FormatMicrotube
Product LineAmbion™, GLOBINclear™
For Use With (Application)Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
Quantity20 Preps
Unit Size20 preps
Contents & Storage
• 20 μl Capture Oligo Mix (-20°C)
• 600 μl Streptavidin Magnetic Beads (4°C—do not freeze)
• 1.5 ml 2X Hybridization Buffer (room temperature)
• 1.5 ml Streptavidin Bead Buffer (room temperature)
• 1.75 Nuclease-free Water (-20°C, 4°C, or room temperature)
• 200 μl RNA Binding Beads (4°C—do not freeze)
• 5 ml RNA Wash Solution Concentrate (4°C or room temperature)
• 1 ml Elution Buffer (4°C or room temperature)
• 80 μl RNA Bead Buffer (room temperature)
• 4 ml RNA Binding Buffer Concentrate (room temperature)
• 100 1.5-ml Non-stick Tubes (room temperature)
• 600 μl Streptavidin Magnetic Beads (4°C—do not freeze)
• 1.5 ml 2X Hybridization Buffer (room temperature)
• 1.5 ml Streptavidin Bead Buffer (room temperature)
• 1.75 Nuclease-free Water (-20°C, 4°C, or room temperature)
• 200 μl RNA Binding Beads (4°C—do not freeze)
• 5 ml RNA Wash Solution Concentrate (4°C or room temperature)
• 1 ml Elution Buffer (4°C or room temperature)
• 80 μl RNA Bead Buffer (room temperature)
• 4 ml RNA Binding Buffer Concentrate (room temperature)
• 100 1.5-ml Non-stick Tubes (room temperature)