The NuPAGE Tris-Acetate Resupply Pack contains:

• NuPAGE Tris-Acetate gels (10 boxes, 100 gels)
• NuPAGE Tris-Acetate Running Buffer for 100 gel runs, 20X (4 bottles, Cat. No. LA0041)
• HiMark Pre-stained Protein Standard for 100 gel runs, 30 to 460 kDa (4 vials, Cat. No. LC5699)

About NuPAGE Tris-Acetate Gels

Invitrogen NuPAGE Tris-Acetate protein gels are precast polyacrylamide gels specifically engineered to provide excellent separation of large molecular weight proteins. These gels do not contain SDS, enabling you to run your proteins in native or denatured form, offering maximum flexibility for diverse protein electrophoresis needs. The unique buffer formulation of these gels maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins when compared to traditional Tris-glycine SDS-PAGE gels.

Not only do NuPAGE Tris-Acetate gels offer high-resolution separation, but they are also designed to ensure optimal transfer of larger molecular weight proteins, making them a preferred choice for researchers performing western blotting. These gels represent a significant advancement over traditional gels, providing both superior performance and flexibility for protein gel electrophoresis and transfer.

Separation

For running Invitrogen protein gels, you have the flexibility to use either the Mini Gel Tank (A25977) or the XCell SureLock Mini-Cell (EI0001). Both systems are designed to provide optimal performance. The Mini Gel Tank's unique side-by-side design offers efficient and convenient electrophoresis for both precast and SureCast handcast mini gels. Alternatively, the XCell SureLock Mini-Cell allows quick, easy, and leak-free running of mini gels, without the need for any clamps. This compatibility gives you the freedom to choose the best tool for your specific lab and experiment requirements. Additionally, the Mini Gel Tank is conveniently available through Invitrogen Precast Gel Welcome Packs along with both sample buffer and reducing agent, providing an all-in-one solution for your electrophoresis needs.

Transfer

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer (NP0006) for traditional wet transfer using the Mini Blot Module (B1000). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).