The Oncomine IGH FR2-J Assay is a targeted next-generation sequencing (NGS) assay for detection of B-cell diversity for clonality assessment to measure clonal expansion with accuracy and for detection of rare clones with high sensitivity. Primers have been specifically designed to regions of the immunoglobulin heavy chain with lower levels of somatic hypermutation to increase primer-binding efficiency. The Oncomine IGH FR2-J Assay is an ideal solution when additional resolution is required following an initial test using an assay such as the Oncomine BCR Pan-Clonality Assay (A51559) or Oncomine BCR IGH-LR Assay (A45485). When combined with Ion Oncomine Reporter Software, you can easily analyze your data and create reports within a short period of time.

Key features of the Oncomine IGH FR2-J Assay:
• Sequencing of FR2-J region of IGH chain
• Detects rare clones at LOD of 10^-6
• Optimized Oncomine informatics for easy, all-inclusive analysis and publication-ready data plots

The kit provides reagents for library construction and includes dNTPs. Ion AmpliSeq libraries can be generated from DNA extracted from fresh/frozen tissue, bone marrow, whole blood, peripheral blood leukocytes, PBMCs, and sorted B cells with as little as 50 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 System with the Ion 530 and Ion 540 chips to enable up to 18 samples per run.

When the primary test isn’t enough
Often when the desired results are not achieved with a primary test, an assay designed with primers directed to different regions of the V(D)J region can be used as a secondary or follow-up test to improve sensitivity of detection. Where needed, secondary testing of samples can be achieved using the Oncomine IGH FR2-J Assay.