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Gibco™
DMEM/F-12
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many differentRead more
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| Catalog Number | Quantity |
|---|---|
| 11320033 | 500 mL |
| 11320082 | 10 x 500 mL |
Catalog number 11320033
Price (CNY)
520.60
Precio exclusivo en nuestra web
Ends: 31-Dec-2025
664.00Save 143.40 (22%)
Each
-
Quantity:
500 mL
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
520.60
Precio exclusivo en nuestra web
Ends: 31-Dec-2025
664.00Save 143.40 (22%)
Each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineMDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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