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Gibco™
TrypLE™ Express Enzyme (1X), phenol red
TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO,Read more
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Catalog Number
Quantity
12605028
500 mL
12605010
100 mL
12605036
20 x 100mL
12605093
5 L
4 Options
Catalog number 12605028
Price (CNY)
1,055.00
Each
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500 mL
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Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas. Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas. Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
1,055.00
Each
Add to cart
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TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.
We offer a variety of TrypLE formulations for a range of cell culture applications.
Compared to trypsin and other dissociation reagents, Gibco TrypLE Express is:
Gentle on cells
• Room-temperature stable • Easy to use • Animal origin-free (AOF)
This TrypLE Express is modified as follows:
With
• Phenol Red
• EDTA
The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.
Gentle On Cells TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.
Room-Temperature Stable TrypLE Express is room-temperature stable and ready to use. TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to Use TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin-Free (AOF) Unlike porcine trypsin, TrypLE Express is free of animal-derived components.
cGMP Manufacturing and Quality System For supply chain continuity, we manufacture Gibco TrypLE Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1 X
Culture TypeAdherent Cell Culture
Green FeaturesEnergy efficient, Sustainable packaging
OsmolalityCheck and Record
Product LineGibco™
Quantity500 mL
RemarksRoom Temperature Stable
Shelf Life24 Months
Shipping ConditionRoom Temperature
SourceAnimal origin-free
Tested ForIn vitro Bioassay
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
With AdditivesPhenol Red, EDTA
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C, protect from light Alternate storage conditions: 2°C to 8°C or -5°C to -20°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture
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Frequently asked questions (FAQs)
How can I assess residual TrypLE reagent remaining after my downstream processing steps?
We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.
Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?
There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.
For your TrypLE products, how does the enzyme get inactivated?
Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:
After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.
What is the difference between TrypLE Express Enzyme, with and without phenol red? What does the phenol red do?
The difference between the two products is just the presence or absence of phenol red. Phenol red is a pH indicator. You can use either product to dissociate your cells. When you use TrypLE Express enzyme to dissociate cells, they very likely become single cells. If you want to passage cells as clumps, you may consider using other reagents such as EDTA.