Dispase II, powder

Try new Gibco Insulin-Transferrin-Selenium Select supplements, an animal-origin free formulation. Available in ITS-G & ITS-X. Learn more >

Gibco™

Dispase II, powder

Dispase II (neutral protease) is an amino-endo peptidase that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. ThisRead more

Catalog Number	Quantity
17105041	5 g

Catalog number 17105041

Price (CNY)

15,013.00

Each

Add to cart

Quantity:

5 g

Delivery information

Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.

Price (CNY)

15,013.00

Each

Add to cart

Dispase II (neutral protease) is an amino-endo peptidase that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. This sequence is found with high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. Dispase II is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity.

Gibco™ Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and to prevent clumping in suspension cultures. Gibco™ Dispase II activity is guaranteed to be greater than 0.5 units/mg.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity5 g

Shelf Life24 Months

Shipping ConditionRoom Temperature

FormLyophilized

Product TypeCell Culture Dissociation Reagent

SterilityNon-sterile

Unit SizeEach

Contents & Storage

Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

What is the stability of reconstituted Dispase?

The Dispase solution is stable at -5 to -20 degrees C for a minimum of 1 month.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I have Dispase, Cat. No. 17105041. Is it Dispase I or II?

This product is Dispase II.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why is collagenase type IV favored over dispase even though the dissociation with collagenase IV seems to take longer (between 30 and 60 min, depending from the lot, at 37 degrees C) compared to dispase?

Actually, in a feeder-based culture, dispase (2 mg/mL) should take about 15-25 min to work at 37 degrees C. Two to three minutes' dissociation time would apply to feeder-free cultures. Dispase is a more aggressive enzyme, so it works faster, but that also means that when the PSC clumps are harvested, they are more sensitive to being broken apart by trituration. Once the clumps are harvested, they should be pipetted up and down a few times to break up the clumps to the appropriate size. If the cells are harvested with collagenase type IV, they have to be pipetted more times because the clumps are harder to break up, but this means that there is less likelihood to break up the clumps into pieces that are too small. If the cells are harvested with dispase, they have to be pipetted fewer times, and care has to be taken to ensure that the clumps are not broken too much. Either enzyme is fine to use, and if you have enough experience, you may prefer to use dispase to save time. But for a less experienced user, we recommend using collagenase type IV as it is safer and you are less likely to ruin your culture by over-triturating.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.