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Thermo Scientific™
PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250Read more
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| Catalog Number | Quantity |
|---|---|
| 26619 | 2 x 250 μL |
| 26620 | 10 x 250 μL |
Catalog number 26619
Price (CNY)
1,079.00
Each
-
Quantity:
2 x 250 μL
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
1,079.00
Each
Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Applications
- Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring protein transfer onto membranes after western blotting
- Sizing of proteins on SDS-polyacrylamide gels and western blots
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Gel CompatibilityBolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
Product LinePageRuler™
Product TypeProtein Ladder
Quantity2 x 250 μL
Ready to LoadYes
Shipping ConditionApproved for shipment on Wet or Dry Ice
Stain Type3 colors: Blue, Orange, Green
System TypeWestern Blotting, SDS-PAGE
Number of Markers9
Size Range10 to 250 kDa
Unit SizeEach
Contents & Storage
Contents: two vials of 250 μL each
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C
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Frequently asked questions (FAQs)
Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.