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Gibco™
Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumRead more
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| Catalog Number | Quantity |
|---|---|
| 31985062 | 100 mL |
| 31985070 | 500 mL |
| 31985088 | 10 x 500 mL |
Catalog number 31985062
Price (CNY)
281.00
Online Exclusive
Ends: 28-Jun-2025
408.00Save 127.00 (31%)
Each
-
Quantity:
100 mL
Price (CNY)
281.00
Online Exclusive
Ends: 28-Jun-2025
408.00Save 127.00 (31%)
Each
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.
This Opti-MEM™ I is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineSp2, AE-1, CHO, BHK-21, and HEK
Cell TypePrimary Fibroblasts
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, Opti-MEM™
Product TypeOpti-MEM I
Quantity100 mL
Shelf Life18 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationHuman Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red
Unit SizeEach
Contents & Storage
Store at 2–8°C and protect from light.
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Lot #Certificate TypeDateCatalog Number(s)
3225302Certificate of AnalysisJun 27, 202531985047, 31985062, 31985070
3150493Certificate of AnalysisJun 22, 202531985047, 31985062, 31985070
3141618Certificate of AnalysisJun 06, 202531985047, 31985062, 31985070
3141616Certificate of AnalysisJun 06, 202531985047, 31985062, 31985070
3137998Certificate of AnalysisJun 04, 202531985047, 31985062, 31985070
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Frequently asked questions (FAQs)
We recommend using Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Opti-MEM I Reduced Serum Medium, no phenol red (Cat. No. 11058021) would be the closest alternative. Both offerings contain L-glutamine. For more a stabilized L-glutamine option, Opti-MEM I Reduced Serum Medium, GlutaMAX Supplement (Cat. No. 51985034) is the recommended alternative. All Opti-MEM offerings have demonstrated performance for both reduced-serum cell culture and lipid-based transfection.
This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (25)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are
Regulation of cortical dendrite development by Slit-Robo interactions.
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching.
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T
Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection.
Journal:PloS one
PubMed ID:38833479
Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study
Vinexin beta Regulates the Anchorage Dependence of ERK2 Activation Stimulated by Epidermal Growth Factor.
Journal:J Biol Chem
PubMed ID:11825889
'ERK is activated by soluble growth factors in adherent cells. However, activation of ERK is barely detectable and not sufficient for cell proliferation in non-adherent cells. Here, we show that exogenous expression of vinexin beta, a novel focal adhesion protein, allows anchorage-independent ERK2 activation stimulated by epidermal growth factor. In
25 total citations