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Applied Biosystems™
TaqMan™ Gene Expression Master Mix
Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix.Read more
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| Catalog Number | Quantity |
|---|---|
| 4369016 | 1 x 5 mL |
| 4369542 | 10 x 5 mL |
| 4370048 | 1 x 1 mL |
| 4369514 | 2 x 5 mL |
| 4369510 | 5 x 5 mL |
| 4370074 | 1 x 50 mL |
Catalog number 4369016
Price (CNY)
6,180.00
Each
-
Quantity:
1 x 5 mL
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
6,180.00
Each
Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family members
TaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.
Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family members
TaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.
Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
No. of Reactions200 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq Gold DNA Polymerase
Product LineTaqMan™
Purity or Quality GradeUP (Ultra Pure)
Quantity1 x 5 mL
Sample TypeDNA (Genomic), cDNA
Volume1 x 5 mL
Detection MethodPrimer-probe
For Use With (Application)Gene Expression
FormLiquid
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
1 bottle (5 mL) 2X TaqMan™ Gene Expression Master Mix sufficient for 200 reactions. Store at 2°C to 8°C.
TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.
TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.
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Frequently asked questions (FAQs)
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.