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Gibco™
Viral Production Cells
Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (Cat. No. A35684).
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| Catalog Number | Quantity |
|---|---|
| A35827 | 6 x 1 mL |
| A35347CN | 1 x 10^7 cells/1 mL |
| A35347 | 1 mL |
Catalog number A35827
Price (CNY)Contact Us
-
Quantity:
6 x 1 mL
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (A35684). Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium (A3583401). They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent (A35348) and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.
- Suspension culture cells facilitate high-throughput screening or scale-up
- Higher viral production levels than other suspension HEK 293 cells
- Rapid cell growth and high culture viabilities
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.
The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeViral Production Cells
Quantity6 x 1 mL
Shipping ConditionDry Ice
Cell LineViral Production Cell-Line
Culture TypeSuspension Cell Culture
Unit SizeEach
Contents & Storage
Store in liquid nitrogen until ready to use. Do not store the cells at -80°C.
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Frequently asked questions (FAQs)
The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.
The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.
Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.
Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at –80 degrees C.