Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye
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Invitrogen™
Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye
The Click-iT EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays that is optimized forRead more
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Catalog Number
Quantity
C10337
1 kit
Catalog number C10337
Price (CNY)
6,556.00
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Ends: 31-Dec-2025
8,886.00
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Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas. Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas. Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
6,556.00
Online Exclusive
Ends: 31-Dec-2025
8,886.00
Save 2,330.00 (26%)
Each
Add to cart
Ask our AI about this Product
The Click-iT EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.
• Simple—works the first time, every time, in less time • Efficient—no denaturation steps or harsh treatment required • Content-rich results—better preservation of cell morphology, antigen structure, and DNA integrity • Consistent—not dependent on variable antibody lots for detection
The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue. BrdU-labeled DNA is quantitated using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules. This step is time consuming and difficult to perform consistently. The harsh treatment can also adversely effect sample integrity and quality, which makes co-staining with other antibodies challenging.
Superior Proliferation Methodology The Click-iT EdU Cell Proliferation Kit for Imaging provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT™ EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT™ EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT™ EdU imaging kit is not only easy to use, but more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.
This kit is optimized for fluorescence microscopy applications; visit the Click-iT™ technology area of our website for kits designed for high-content imaging, fluorescence microplate, or flow cytometry platforms.
Notes: The Click-iT™ assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods. The Click-iT™ assay can be used with BrdU in dual pulse experiments by using the anti-BrdU (clone MoBu-1) antibody, which does not cross react with EdU. The Click-iT™ technology is compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeAlexa Fluor™ 488
FormatSlide(s)
Green FeaturesLess hazardous
Quantity1 kit
Shipping ConditionRoom Temperature
ColorGreen
EmissionVisible
For Use With (Application)Proliferation Assay
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope
Each kit contains enough material for 50 to 250 reactions, depending on reaction volume (typically between 0.1 and 0.5 ml). Store at 2–6°C, dessicate and protect from light.
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Frequently asked questions (FAQs)
I will be performing a cell proliferation assay using Click-iT EdU kit. At what point can I stop overnight, or do I have to perform all the steps continuously?
One may store the sample after fixation overnight in PBS at 4oC. For longer storage (<1 week) , store in buffer with 1-2% formaldehyde or in formalin to limit microbial growth. If you use sodium azide as a microbial inhibitor, it must be completely removed prior to the Click-iT reaction.
A control for a Click-iT EdU labeling experiment uses no EdU and the Click-iT reaction using Alexa Fluor 594 azide. The mouse heart tissue sections are showing non-specific labeling in red, seen in particular clusters of cells. They don't overlap with DAPI. What is the problem?
The problem is likely not the Alexa Fluor 594 azide. Since there are no alkynes endogenous to mouse tissue, there is nothing for the dye-azide to bind to. Since the background doesn't overlap with nuclei (DAPI signal), this isn't an issue of unintended EdU labeling. This red is autofluorescence from red blood cells; they autofluoresce in the red and don't have nuclei. This can be confirmed by checking a completely unlabeled tissue section (no dye present at all) to see if they are still present and by examining the cells at high magnification and looking for corpuscular shape.
Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?
We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.
For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.
Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.
Are the Alexa Fluor azides from Click-iT EdU kits available separately?
Yes, but the standalone products are not shipped at the same amount as provided in the Click-iT EdU kits; the amount of dye-azide provided in the Click-iT kits is proprietary information. See these catalog numbers for the standalone products:
- Cat. No. A10266: Alexa Fluor 488 azide
- Cat. No. A10270: Alexa Fluor 594 azide
- Cat. No. A10277: Alexa Fluor 647 azide
I am observing no signal or very low specific signal for my click-labeled samples. What can I do to improve the signal?
The click reaction is only effective when copper is in the appropriate valency. Azides and alkynes will not react with each other without copper. Make sure that the click reaction mixture is used immediately after preparation when the copper (II) concentration is at its highest.
Do not use additive buffer that has turned yellow; it must be colorless to be active.
Cells need to be adequately fixed and permeabilized for the TdT enzyme and click reagents to have access to the nucleus. Tissue samples require digestion with proteinase K or other proteolytic enzymes for sufficient TdT access.
Some reagents can bind copper and reduce its effective concentration available to catalyze the click reaction. Do not include any metal chelator (e.g., EDTA, EGTA, citrate, etc.) in any buffer or reagent prior to the click reaction. Avoid buffers or reagents that include other metal ions that may be o xidized or reduced. It may be help to include extra wash steps on the cell or tissue sample before performing the click reaction.
You can repeat the click reaction with fresh reagents to try to improve signal. Increasing the click reaction time longer than 30 minutes will not improve a low signal. Performing a second, 30 minute incubation with fresh click reaction reagents is more effective at improving labeling.
Your cells may not be apoptotic. Prepare a DNase I-treated positive control to verify that the TdT enzymatic reaction and click labeling reaction are working correctly.