EnzChek™ Lysozyme Assay Kit
EnzChek™ Lysozyme Assay Kit
Invitrogen™

EnzChek™ Lysozyme Assay Kit

The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. TheRead more
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Catalog NumberQuantity
E22013400 assays
Catalog number E22013
Price (CNY)
5,256.00
Each
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Quantity:
400 assays
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
5,256.00
Each
Add to cart
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The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader.

See our complete line of Fluorescence Microplate assays.

• Detect lysozyme activity concentrations as low as 20 U/mL
• Use standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of lysozyme activity

The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence Intensity
Quantity400 assays
Shipping ConditionRoom Temperature
Substrate PropertiesLipid-Based Substrate
Substrate TypeLysozyme Substrate
Target EnzymeLysozyme
For Use With (Application)Lysozyme Assay
For Use With (Equipment)Fluorescence Microplate Reader
Product LineEnzChek
Product TypeLysozyme Assay
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
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Frequently asked questions (FAQs)

How can I limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013)?

To limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013), try lowering the voltage (gain) on the PMT (photomultiplier tube). If you are still seeing signal saturation after lowering the voltage, try a shorter reaction time, and/or lower substrate concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?

The primary amines on the cell wall of Micrococcus lysodeikticus were conjugated to fluorescein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May other buffers be used for the EnzChek Lysozyme assay?

Yes. You may use other buffers that are compatible with the enzyme of interest. The buffer provided is optimal for use with chicken egg white lysozyme. If the buffer has a pH <6, adjust the pH to 7.5 to 8 prior to detection for optimal signal from the fluorescein. The emission of fluorescein drops off at pH <6.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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