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NuPAGE™ Transfer Buffer (20X)
NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for westernRead more
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| Catalog Number | Quantity |
|---|---|
| NP00061 | 1L |
| NP0006 | 125mL |
Catalog number NP00061
Price (CNY)
1,408.00
Online Exclusive
Ends: 31-Dec-2025
2,131.00Save 723.00 (34%)
Each
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Quantity:
1L
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
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Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (CNY)
1,408.00
Online Exclusive
Ends: 31-Dec-2025
2,131.00Save 723.00 (34%)
Each
NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.
NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes.
See all available buffers and reagents available for SDS-PAGE
NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes.
See all available buffers and reagents available for SDS-PAGE
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferTransfer Buffers
Concentration20 X
Gel CompatibilityNuPAGE™ Gels
Product LineNuPAGE™
Product TypeTransfer Buffer
Quantity1L
Shelf Life6 Months
Shipping ConditionRoom Temperature
Gel TypeNuPAGE
Unit SizeEach
Contents & Storage
Store at room temperature. Guaranteed stable for 6 months unless otherwise specified in the product literature.
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Frequently asked questions (FAQs)
DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Yes. The NuPAGE transfer buffer works with Tris-Glycine gels. Proteins are subjected to a more neutral pH, and the absence of glycine in the NuPAGE transfer buffer makes protein sequencing of proteins extracted from the gels much easier. While protein transfer is generally more efficient when using the NuPAGE transfer buffer, remember that the other benefits of the NuPAGE system (band sharpness, better resolution, sample stability) only apply if the proteins have been separated under these conditions prior to transfer (i.e., on a NuPAGE gel).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.